气候变化领域集成服务门户(CCPortal): TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line

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DOI	10.3389/fpls.2021.762265
	TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line
	Liu, Xin; Zhang, Minghu; Jiang, Xiaomei; Li, Hui; Jia, Zhenjiao; Hao, Ming; Jiang, Bo; Huang, Lin; Ning, Shunzong; Yuan, Zhongwei; Chen, Xuejiao; Chen, Xue; Liu, Dengcai; Liu, Baolong; Zhang, Lianquan
通讯作者	Zhang, LQ (通讯作者)，Sichuan Agr Univ, Triticeae Res Inst, Chengdu, Peoples R China. ; Zhang, LQ (通讯作者)，Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat South, Chengdu, Peoples R China. ; Liu, BL (通讯作者)，Qinghai Prov Key Lab Crop Mol Breeding, Xining, Peoples R China. ; Liu, BL (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining, Peoples R China.
发表日期	2021
ISSN	1664-462X
卷号	12
英文摘要	Triticum boeoticum Boiss (A(b)A(b), 2n = 2x = 14) is one of the sources of the blue grain trait controlled by blue aleurone layer 2 (Ba2). However, the underlying genes have not been cloned. In this study, a transcriptomic comparison between a blue-grained wheat-T. boeoticum substitution line and its wheat parent identified 41 unigenes related to anthocyanin biosynthesis and 29 unigenes related to transport. The bHLH transcription factor gene TbMYC4A showed a higher expression level in the blue-grained substitution line. TbMYC4A contained the three characteristic bHLH transcription factor domains (bHLH-MYC_N, HLH and ACT-like) and clustered with genes identified from other wheat lines with the blue grain trait derived from other Triticeae species. TbMYC4A overexpression confirmed that it was a functional bHLH transcription factor. The analysis of a TbMYC4A-specific marker showed that the gene was also present in T. boeoticum and T. monococcum with blue aleurone but absent in other Triticeae materials with white aleurone. These results indicate that TbMYC4A is a candidate gene of Ba2 controlling the blue aleurone trait. The isolation of TbMYC4A is helpful for further clarifying the genetic mechanism of the blue aleurone trait and is of great significance for breeding blue-grained wheat varieties.
关键词	DIPLOID WHEAT ANTHOCYANIN BIOSYNTHESIS EXPRESSION PURPLE COLOR PIGMENTS BLACK MAP
英文关键词	Triticum boeoticum; anthocyanin biosynthesis; blue aleurone; transcriptome analysis; TbMYC4A
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000720862800001
来源期刊	FRONTIERS IN PLANT SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254451
作者单位	[Liu, Xin; Zhang, Minghu; Jiang, Xiaomei; Li, Hui; Jia, Zhenjiao; Hao, Ming; Jiang, Bo; Huang, Lin; Ning, Shunzong; Yuan, Zhongwei; Chen, Xuejiao; Chen, Xue; Liu, Dengcai; Zhang, Lianquan] Sichuan Agr Univ, Triticeae Res Inst, Chengdu, Peoples R China; [Huang, Lin; Liu, Dengcai; Zhang, Lianquan] Sichuan Agr Univ, State Key Lab Crop Gene Explorat & Utilizat South, Chengdu, Peoples R China; [Liu, Baolong] Qinghai Prov Key Lab Crop Mol Breeding, Xining, Peoples R China; [Liu, Baolong] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xin,Zhang, Minghu,Jiang, Xiaomei,et al. TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line[J]. 中国科学院西北生态环境资源研究院,2021,12.
APA	Liu, Xin.,Zhang, Minghu.,Jiang, Xiaomei.,Li, Hui.,Jia, Zhenjiao.,...&Zhang, Lianquan.(2021).TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line.FRONTIERS IN PLANT SCIENCE,12.
MLA	Liu, Xin,et al."TbMYC4A Is a Candidate Gene Controlling the Blue Aleurone Trait in a Wheat-Triticum boeoticum Substitution Line".FRONTIERS IN PLANT SCIENCE 12(2021).