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| DOI | 10.3390/separations8120238 |
| A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay | |
| Wang, Xiu; Zhang, Yupei; Wu, Nan; Cao, Jingya; Tao, Yanduo; Yu, Ruitao | |
| 通讯作者 | Yu, RT (通讯作者)，Northwest Inst Plateau Biol, Chinese Acad Sci, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining 810008, Peoples R China. |
| 发表日期 | 2021 |
| EISSN | 2297-8739 |
| 卷号 | 8期号:12 |
| 英文摘要 | Free radicals, including 1,1-diphenyl-2-picrylhydrazyl, mediate oxidative stress to cause many chronic diseases (including cardiovascular diseases, diabetes and cancer). The extract of traditional Tibetan medicine Lepidium latifolium L. (L. latifolium) was reported to have free radical inhibition ability. Therefore, a system method was established to separate the ethanol extract of L. latifolium to prepare two main antioxidant compounds. First of all, silica gel and a medium-pressure liquid chromatography tower were used for pre-treatment of the ethanol extract of L. latifolium to obtain the main antioxidant active component fraction 4 through online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl (HPLC-DPPH) assay. Then, fraction 4-1 was obtained by one-dimensional preparation using Megres C18 chromatographic column, and two active compounds with IC50 values 59.9 and 71.3 mu g/mL were obtained by two-dimensional preparation using Click XIon chromatographic column. Through the study of the chemical components and separation methods of L. latifolium, the combination of HPLC-DPPH assay and two-dimensional preparative liquid chromatography was realized, providing a reference for the separation of active compounds from L. latifolium. |
| 关键词 | OXIDATIVE STRESSPREPARATIVE ISOLATIONMECHANISMSIDENTIFICATIONDISEASE |
| 英文关键词 | antioxidant activity; Lepidium latifolium L; medium-pressure liquid chromatography; online HPLC-DPPH assay |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Analytical |
| WOS记录号 | WOS:000738672500001 |
| 来源期刊 | SEPARATIONS
 |
| 来源机构 | 中国科学院西北生态环境资源研究院 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/254396 |
| 作者单位 | [Wang, Xiu; Zhang, Yupei; Wu, Nan; Cao, Jingya; Tao, Yanduo; Yu, Ruitao] Northwest Inst Plateau Biol, Chinese Acad Sci, Qinghai Prov Key Lab Tibetan Med Res, Key Lab Tibetan Med Res, Xining 810008, Peoples R China; [Wang, Xiu; Zhang, Yupei; Wu, Nan; Cao, Jingya] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiu,Zhang, Yupei,Wu, Nan,et al. A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay[J]. 中国科学院西北生态环境资源研究院,2021,8(12). |
| APA | Wang, Xiu,Zhang, Yupei,Wu, Nan,Cao, Jingya,Tao, Yanduo,&Yu, Ruitao.(2021).A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay.SEPARATIONS,8(12). |
| MLA | Wang, Xiu,et al."A Method to Separate Two Main Antioxidants from Lepidium latifolium L. Extracts Using Online Medium Pressure Chromatography Tower and Two-Dimensional Inversion/Hydrophobic Interaction Chromatography Based on Online HPLC-DPPH Assay".SEPARATIONS 8.12(2021). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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