气候变化领域集成服务门户(CCPortal): MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1

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DOI	10.3389/fgene.2021.640807
	MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1
	Yang, Lingzhi; Qi, Qi; Wang, Jian; Song, Chengchuang; Wang, Yanhong; Chen, Xi; Chen, Hong; Zhang, Chunlei; Hu, Linyong; Fang, Xingtang
通讯作者	Zhang, CL ; Fang, XT (通讯作者)，Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou, Jiangsu, Peoples R China.
发表日期	2021
EISSN	1664-8021
卷号	12
英文摘要	microRNAs are a kind of endogenous, non-coding, single-strand small RNA. They have been reported as an important regulatory factor in skeletal myogenesis. In this study, miR-452 was selected from RNA high-throughput sequencing data to explore its regulatory role in myogenesis. Functionally, miR-452 overexpression could promote C2C12 myoblast proliferation while inhibiting myogenic differentiation. On the contrary, inhibition of miR-452 could suppress C2C12 myoblast proliferation but accelerate myogenic differentiation. Bioinformatics analysis and dual luciferase report assays showed that Angiopoietin 1 (ANGPT1), RB1, and CACNB4 were the potential target genes of miR-452. To further confirm the target relationship between ANGPT1, RB1, and CACNB4 with miR-452, the mRNA level and protein level of these genes were detected by using RT-qPCR and Western blot, respectively. Result analysis indicated that ANGPT1 was a target gene of miR-452. In addition, knockdown of ANGPT1 could obviously promote C2C12 myoblast proliferation but block their differentiation. In summary, these results demonstrated that miR-452 promoted C2C12 myoblast proliferation and inhibited their differentiation via targeting ANGPT1.
英文关键词	miR-452; proliferation; differentiation; ANGPT1
语种	英语
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000632859800001
来源期刊	FRONTIERS IN GENETICS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254313
作者单位	[Yang, Lingzhi; Qi, Qi; Wang, Jian; Song, Chengchuang; Wang, Yanhong; Chen, Xi; Chen, Hong; Zhang, Chunlei; Fang, Xingtang] Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou, Jiangsu, Peoples R China; [Hu, Linyong] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China
推荐引用方式 GB/T 7714	Yang, Lingzhi,Qi, Qi,Wang, Jian,et al. MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1[J]. 中国科学院西北生态环境资源研究院,2021,12.
APA	Yang, Lingzhi.,Qi, Qi.,Wang, Jian.,Song, Chengchuang.,Wang, Yanhong.,...&Fang, Xingtang.(2021).MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1.FRONTIERS IN GENETICS,12.
MLA	Yang, Lingzhi,et al."MiR-452 Regulates C2C12 Myoblast Proliferation and Differentiation via Targeting ANGPT1".FRONTIERS IN GENETICS 12(2021).