气候变化领域集成服务门户(CCPortal): Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol

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DOI	10.1016/j.snb.2021.129443
	Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol
	Wang, Lulu; Zhang, Han; Su, Lihong; Yao, Xiaolin; Wang, Zonghan; Zhao, Man; Sun, Jing; Wang, Jianlong; Zhang, Daohong
通讯作者	Zhang, DH (通讯作者)，Northwest A&F Univ, Coll Food Sci & Engn, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China.
发表日期	2021
EISSN	0925-4005
卷号	331
英文摘要	Exploring a nanomaterial simultaneously possessing simple synthesis process and brighter colors as a novel label is urgently needed in the immunochromatography assay (ICA). Herein, in order to achieve high-sensitive detection of small molecule pollutants, an immunosensor is developed, where the mild resorcinol formaldehyde resin polymer (mRF, 380 nm) synthesized under mild conditions, was applied as a signal label. The mRF possesses good biocompatibility, various chemical groups, and is easy to bind biological molecules. The antibody was connected to the mRF through electrostatic adsorption to prepare immune probes that was further applied in ICA. In this work, clenbuterol (CL) was chosen as the analysis model to prove the performance of the mRF-based immune probe. Under best conditions, the detection sensitivity is 1 ng mL(-1), that is 4 times lower than that of as much sensitive as the traditional colloidal gold strip. In addition, this method was successfully applied to detect CL in food samples, with recovery rates between 96.7 % and 117.2 %. These results show that the mRF-based ICA is a reliable and sensitive detection tool for the CL residue. This work offers a new possibility for the application of RF and a new reference method for the analysis and detection of other small molecule pollutants.
英文关键词	Immunochromatography assay; Mild reaction condition; Resorcinol formaldehyde resin; Clenbuterol
语种	英语
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000618768500001
来源期刊	SENSORS AND ACTUATORS B-CHEMICAL
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254264
作者单位	[Wang, Lulu; Zhang, Han; Su, Lihong; Yao, Xiaolin; Wang, Zonghan; Zhao, Man; Wang, Jianlong; Zhang, Daohong] Northwest A&F Univ, Coll Food Sci & Engn, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China; [Sun, Jing] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Lulu,Zhang, Han,Su, Lihong,et al. Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol[J]. 中国科学院西北生态环境资源研究院,2021,331.
APA	Wang, Lulu.,Zhang, Han.,Su, Lihong.,Yao, Xiaolin.,Wang, Zonghan.,...&Zhang, Daohong.(2021).Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol.SENSORS AND ACTUATORS B-CHEMICAL,331.
MLA	Wang, Lulu,et al."Mild resorcinol formaldehyde resin polymer based immunochromatography assay for high-sensitive detection of clenbuterol".SENSORS AND ACTUATORS B-CHEMICAL 331(2021).