气候变化领域集成服务门户(CCPortal): Predicted gene 31453 (Gm31453) and the gene encoding carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis and male fertility in the mouse

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DOI	10.1071/RD20284
	Predicted gene 31453 (Gm31453) and the gene encoding carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis and male fertility in the mouse
	Zhou, Yang; Zhang, Xiaona; Xiong, Suping; Zeng, Xuhui; Zhang, Xiaoning
通讯作者	Zeng, XH ; Zhang, XN (通讯作者)，Nantong Univ, Med Sch, Inst Reprod Med, Nantong 226019, Peoples R China. ; Zhang, XN (通讯作者)，Nanchang Univ, Inst Life Sci, Nanchang 330031, Jiangxi, Peoples R China.
发表日期	2021
ISSN	1031-3613
EISSN	1448-5990
起始页码	401
结束页码	409
卷号	33 期号:6
英文摘要	Numerous long non-coding (lnc) RNAs are highly enriched or exclusively expressed in the mammalian testis, even in spermatids. Spermatid perinuclear RNA-binding protein (STRBP) can bind many RNAs, and loss of STRBP impairs male fertility. However, the functions of lncRNAs interacting with STRBP are unknown. In this study, the roles of one STRBP-interacting lncRNA, namely predicted gene 31453 (Gm31453), and its potential target gene encoding carboxypeptidase A5 (Cpa5) in spermatogenesis were determined using gene-knockout (KO) mice. Gm31453 and Cpa5 are located adjacent to each other on the same chromosome and are highly expressed in the testis. Gm31453 and Cpa5 are primarily expressed from secondary spermatocytes to elongated spermatids, implying their involvement in spermiogenesis. Although deletion of Gm31453 disturbed the expression of both its target and interacting gene, as indicated by decreased Cpa5 and increased Strbp mRNA levels, both Gm31453- and Cpa5-KO mice showed normal spermatogenesis and fertility, and had no detectable abnormalities in terms of testicular and epididymal development, sperm production morphology or motility, pregnancy rate or litter size. Thus, Gm31453 and Cpa5 are dispensable for spermatogenesis and male fertility in mice. Their involvement in spermatogenesis may be a fine-tuning role, regulating gene expression at the molecular level.
英文关键词	Cpa5; Gm31453; lncRNA; male fertility; spermatogenesis; Strbp
语种	英语
WOS研究方向	Developmental Biology ; Reproductive Biology ; Zoology
WOS类目	Developmental Biology ; Reproductive Biology ; Zoology
WOS记录号	WOS:000631041600001
来源期刊	REPRODUCTION FERTILITY AND DEVELOPMENT
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254259
作者单位	[Zhou, Yang; Zeng, Xuhui; Zhang, Xiaoning] Nantong Univ, Med Sch, Inst Reprod Med, Nantong 226019, Peoples R China; [Zhang, Xiaona] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810001, Peoples R China; [Xiong, Suping; Zhang, Xiaoning] Nanchang Univ, Inst Life Sci, Nanchang 330031, Jiangxi, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Yang,Zhang, Xiaona,Xiong, Suping,et al. Predicted gene 31453 (Gm31453) and the gene encoding carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis and male fertility in the mouse[J]. 中国科学院西北生态环境资源研究院,2021,33(6).
APA	Zhou, Yang,Zhang, Xiaona,Xiong, Suping,Zeng, Xuhui,&Zhang, Xiaoning.(2021).Predicted gene 31453 (Gm31453) and the gene encoding carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis and male fertility in the mouse.REPRODUCTION FERTILITY AND DEVELOPMENT,33(6).
MLA	Zhou, Yang,et al."Predicted gene 31453 (Gm31453) and the gene encoding carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis and male fertility in the mouse".REPRODUCTION FERTILITY AND DEVELOPMENT 33.6(2021).