气候变化领域集成服务门户(CCPortal): Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice

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DOI	10.1186/s12864-021-07731-x
	Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice
	Wang, Haijing; Liu, Daoxin; Song, Pengfei; Jiang, Feng; Chi, Xiangwen; Zhang, Tongzuo
通讯作者	Zhang, TZ (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China. ; Zhang, TZ (通讯作者)，Qinghai Prov Key Lab Anim Ecol Genom, Xining 810008, Qinghai, Peoples R China.
发表日期	2021
ISSN	1471-2164
卷号	22 期号:1
英文摘要	BackgroundThe spleen is the largest secondary lymphoid organ and the main site where stress erythropoiesis occurs. It is known that hypoxia triggers the expansion of erythroid progenitors; however, its effects on splenic gene expression are still unclear. Here, we examined splenic global gene expression patterns by time-series RNA-seq after exposing mice to hypoxia for 0, 1, 3, 5, 7 and 13days.ResultsMorphological analysis showed that on the 3rd day there was a significant increase in the spleen index and in the proliferation of erythroid progenitors. RNA-sequencing analysis revealed that the overall expression of genes decreased with increased hypoxic exposure. Compared with the control group, 1380, 3430, 4396, 3026, and 1636 genes were differentially expressed on days 1, 3, 5, 7 and 13, respectively. Clustering analysis of the intersection of differentially expressed genes pointed to 739 genes, 628 of which were upregulated, and GO analysis revealed a significant enrichment for cell proliferation. Enriched GO terms of downregulated genes were associated with immune cell activation. Expression of Gata1, Tal1 and Klf1 was significantly altered during stress erythropoiesis. Furthermore, expression of genes involved in the immune response was inhibited, and NK cells decreased.ConclusionsThe spleen of mice conquer hypoxia exposure in two ways. Stress erythropoiesis regulated by three transcription factors and genes in immune response were downregulated. These findings expand our knowledge of splenic transcriptional changes during hypoxia.
关键词	EXTRAMEDULLARY HEMATOPOIESIS TRANSCRIPTION EXPRESSION LACKING PACKAGE CELLS POLYCYTHEMIA RESERVOIR PROMOTER KINASE
英文关键词	Transcriptome; Spleen; Stress erythropoiesis; Immune response; Hypoxia
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000660893500001
来源期刊	BMC GENOMICS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254224
作者单位	[Wang, Haijing; Liu, Daoxin; Song, Pengfei; Jiang, Feng; Chi, Xiangwen; Zhang, Tongzuo] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China; [Wang, Haijing] Qinghai Univ, Med Coll, Xining 810016, Qinghai, Peoples R China; [Wang, Haijing; Liu, Daoxin; Song, Pengfei; Jiang, Feng; Chi, Xiangwen; Zhang, Tongzuo] Qinghai Prov Key Lab Anim Ecol Genom, Xining 810008, Qinghai, Peoples R China; [Wang, Haijing; Liu, Daoxin; Song, Pengfei; Jiang, Feng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Haijing,Liu, Daoxin,Song, Pengfei,et al. Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice[J]. 中国科学院西北生态环境资源研究院,2021,22(1).
APA	Wang, Haijing,Liu, Daoxin,Song, Pengfei,Jiang, Feng,Chi, Xiangwen,&Zhang, Tongzuo.(2021).Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice.BMC GENOMICS,22(1).
MLA	Wang, Haijing,et al."Exposure to hypoxia causes stress erythropoiesis and downregulates immune response genes in spleen of mice".BMC GENOMICS 22.1(2021).