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DOI	10.1093/chromsci/bmaa094
	HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent
	Yang, Xue; Liu, Yongling; Chen, Tao; Wang, Nana; Li, Hongmei; Zhao, Jingyang; Li, Yulin
通讯作者	Chen, T (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, 52 Sanlihe Rd, Xining 810001, Peoples R China.
发表日期	2021
ISSN	0021-9665
EISSN	1945-239X
起始页码	175
结束页码	181
卷号	59 期号:2
英文摘要	Separation of natural compounds directly from the crude extract is a challenging work for traditional column chromatography. In the present study, an efficient method for separation of three main compounds from the crude extract of Dracocephalum tanguticum has been successfully established by high-speed counter-current chromatography (HSCCC). The crude extract was directly introduced into HSCCC by using dimethyl sulfoxide as cosolvent. Ethyl acetate/n-butyl alcohol/0.3% glacial acetic acid (4: 1: 5, v/v) system was used and three target compounds with purity higher than 80% were obtained. Preparative HPLC was used for further purification and three target compounds with purity higher than 98% were obtained. The compounds were identified as chlorogenic acid, pedaliin and pedaliin-6 ''-acetate.
关键词	CHROMATOGRAPHY
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000613898300010
来源期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254121
作者单位	[Yang, Xue; Chen, Tao; Wang, Nana; Li, Hongmei; Zhao, Jingyang; Li, Yulin] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Tibetan Med Pharmacol & Safety Ev, 52 Sanlihe Rd, Xining 810001, Peoples R China; [Yang, Xue; Wang, Nana] Univ Chinese Acad Sci, Savaid Med Sch, 19B Yuquan Rd, Beijing 100049, Peoples R China; [Liu, Yongling] Guiyang Univ, Food & Pharmaceut Engn Inst, 103 Jianlongdong Rd, Guiyang 550005, Peoples R China
推荐引用方式 GB/T 7714	Yang, Xue,Liu, Yongling,Chen, Tao,et al. HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent[J]. 中国科学院西北生态环境资源研究院,2021,59(2).
APA	Yang, Xue.,Liu, Yongling.,Chen, Tao.,Wang, Nana.,Li, Hongmei.,...&Li, Yulin.(2021).HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent.JOURNAL OF CHROMATOGRAPHIC SCIENCE,59(2).
MLA	Yang, Xue,et al."HSCCC Separation of Three Main Compounds from the Crude Extract of Dracocephalum Tanguticum by Using Dimethyl Sulfoxide as Cosolvent".JOURNAL OF CHROMATOGRAPHIC SCIENCE 59.2(2021).