气候变化领域集成服务门户(CCPortal): Competitive Lateral Flow Immunoassay Relying on Au-SiO2 Janus Nanoparticles with an Asymmetric Structure and Function for Furazolidone Residue Monitoring

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DOI	10.1021/acs.jafc.0c06016
	Competitive Lateral Flow Immunoassay Relying on Au-SiO2 Janus Nanoparticles with an Asymmetric Structure and Function for Furazolidone Residue Monitoring
	Su, Lihong; Wang, Lulu; Xu, Jingke; Wang, Zonghan; Yao, Xiaolin; Sun, Jing; Wang, Jianlong; Zhang, Daohong
通讯作者	Zhang, DH (通讯作者)，Northwest A&F Univ, Coll Food Sci & Engn, Xianyang 712100, Shaanxi, Peoples R China.
发表日期	2021
ISSN	0021-8561
EISSN	1520-5118
起始页码	511
结束页码	519
卷号	69 期号:1
英文摘要	Gold nanoparticles (AuNPs) are the most commonly used signal materials in lateral flow immunoassay (LFIA). However, the assay sensitivity of traditional AuNP-based LFIA is usually limited by the incomplete competition between free target analytes and immobilized antigens for the binding of Au-NP-labeled antibodies. To unfreeze this limitation, here, asymmetric Au-SiO2 Janus NPs (about 66 nm) were designed and synthesized. Au-SiO2 Janus NPs can assemble into snowman-like anisotropic structures and combine two different physicochemical properties at their opposite sides, where the AuNP side mainly possesses the antibody conjugating and signal providing functions and the SiO2 side primarily offers the stable function. In virtue of the unique asymmetric nanostructure, only the AuNP side can interact with target analytes by specific antigen-antibody interactions, which could significantly improve the efficiency of competition. Selecting furazolidone as a model analyte, the immunoassay biosensor showed a limit of detection as low as 0.08 ng/mL, 10-fold decreased than that of the AuNPs-LFIA. Moreover, the Au-SiO2 Janus NP lateral flow immunoassay was well applied in chicken, pork, honey, and beef food samples with visual detection limits of 0.8 ng/g, 0.16 ng/g, 0.4 ng/mL, and 0.16 ng/g, respectively. The Au-SiO2 Janus NPs possess the advantages of both materials, which will broaden their applications as a potential alternative in the rapid and sensitive detection of antibiotic residues.
关键词	MESOPOROUS SILICA NANOCARRIERS SENSITIVE DETECTION GOLD NANOPARTICLES DRUG-DELIVERY ASSAY COMPOSITE NANOSPHERES PARTICLES CONJUGATE DESIGN
英文关键词	Au-SiO2 Janus nanoparticle; asymmetric structure; lateral flow immunoassay; furazolidone
语种	英语
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
WOS类目	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
WOS记录号	WOS:000611076800048
来源期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254113
作者单位	[Su, Lihong; Wang, Lulu; Xu, Jingke; Wang, Zonghan; Yao, Xiaolin; Wang, Jianlong; Zhang, Daohong] Northwest A&F Univ, Coll Food Sci & Engn, Xianyang 712100, Shaanxi, Peoples R China; [Sun, Jing] Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Su, Lihong,Wang, Lulu,Xu, Jingke,et al. Competitive Lateral Flow Immunoassay Relying on Au-SiO2 Janus Nanoparticles with an Asymmetric Structure and Function for Furazolidone Residue Monitoring[J]. 中国科学院西北生态环境资源研究院,2021,69(1).
APA	Su, Lihong.,Wang, Lulu.,Xu, Jingke.,Wang, Zonghan.,Yao, Xiaolin.,...&Zhang, Daohong.(2021).Competitive Lateral Flow Immunoassay Relying on Au-SiO2 Janus Nanoparticles with an Asymmetric Structure and Function for Furazolidone Residue Monitoring.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,69(1).
MLA	Su, Lihong,et al."Competitive Lateral Flow Immunoassay Relying on Au-SiO2 Janus Nanoparticles with an Asymmetric Structure and Function for Furazolidone Residue Monitoring".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 69.1(2021).