气候变化领域集成服务门户(CCPortal): Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography

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DOI	10.1002/jssc.202100546
	Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography
	Wang, Weidong; Zhu, Yunhe; Jiang, Lei; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen
通讯作者	Tao, YD ; Liu, ZG (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China.
发表日期	2021
ISSN	1615-9306
EISSN	1615-9314
起始页码	3967
结束页码	3975
卷号	44 期号:21
英文摘要	The high-polar compounds from natural products are often used as medicines due to their good bioactivities. However, owing to the complexity and diversity of their structure, the separation of high-polar compounds is still a challenging work. For this, an efficient method for enrichment and separation of the high-polar compounds from Saussurea obvallata was developed. First, the target compounds were enriched from the total extract using a solid-phase extraction method. An offline two-dimensional liquid chromatography method was used for the separation of pure compounds from the enriched sample. After optimization of chromatographic conditions, high separation selectivity of target compounds was obtained on a polar-modified C18 column and a HILIC XAmide column. Hence, a two-dimensional reversed-phase x hydrophilic interaction liquid chromatography system was constructed and enlarged from the analytical level to the preparative level. In the first dimension, four fractions were obtained on the XCharge C18 column with a recovery rate of 71.2%. In the second-dimension preparation on the XAmide column, eight high-polar compounds with more than 96% purity were isolated. All compounds were isolated from Saussurea obvallata for the first time. The results demonstrated that this developed strategy is effective for preparative-scale isolation of high-polar compounds from natural products.
关键词	HYDROPHILIC INTERACTION CHEMICAL-CONSTITUENTS PURIFICATION COMPONENTS GLYCOSIDES SAPONINS TIME C18 MS
英文关键词	enrichment; high-polarity compounds; offline two-dimensional liquid chromatography; Saussurea obvallata; solid-phase extraction
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000696403200001
来源期刊	JOURNAL OF SEPARATION SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/254080
作者单位	[Wang, Weidong; Zhu, Yunhe; Jiang, Lei; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China; [Wang, Weidong; Zhu, Yunhe; Jiang, Lei; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining, Peoples R China; [Wang, Weidong; Zhu, Yunhe] Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weidong,Zhu, Yunhe,Jiang, Lei,et al. Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography[J]. 中国科学院西北生态环境资源研究院,2021,44(21).
APA	Wang, Weidong,Zhu, Yunhe,Jiang, Lei,Mei, Lijuan,Tao, Yanduo,&Liu, Zenggen.(2021).Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography.JOURNAL OF SEPARATION SCIENCE,44(21).
MLA	Wang, Weidong,et al."Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography".JOURNAL OF SEPARATION SCIENCE 44.21(2021).