气候变化领域集成服务门户(CCPortal): Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography

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DOI	10.1093/chromsci/bmab095
	Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography
	Chen, Tao; Wang, Shuo; Li, Hongmei; Shen, Cheng; Yan, Shuping; Wei, Yangfei; Song, Zhibo; Li, Peipei; Li, Yulin
通讯作者	Li, YL (通讯作者)，Chinese Acad Sci, Northwest Plateau Inst Biol, Xining 810001, Peoples R China.
发表日期	2020
ISSN	0021-9665
EISSN	1945-239X
英文摘要	The pomace of sea buckthorn berries is usually discarded when transforming into nonalcoholic or alcoholic beverages, jellies, jams, juices, candies and dairy products. Here, we established a promising approach for one step separation of five flavonoids from the waste pomace of sea buckthorn berries through counter-current chromatography. The crude extract of waste pomace of sea buckthorn berries after juicing was injected into counter-current chromatography with hexane/ethyl acetate/ethanol/water (v/v/v/v, 5:7:5:7) as the solvent system. As a result, five flavonoids, including quercetin, laricitrin, isorhamnetin-7-O-rhamnoside, kaempferol and isorhamnetin, were obtained in a single step separation. Our finding showed that ethanol is a good substitute for methanol to regulate the partition coefficient in hexane/ethyl acetate/ methanol/water system. This study provided a significant measure to utilize the waste pomace of Sea buckthorn berries.
关键词	HIPPOPHAE-RHAMNOIDES L.PREPARATIVE SEPARATION LIQUID-CHROMATOGRAPHY PURIFICATION MICE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000756452200001
来源期刊	JOURNAL OF CHROMATOGRAPHIC SCIENCE
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253941
作者单位	[Chen, Tao; Wang, Shuo; Li, Hongmei; Shen, Cheng; Yan, Shuping; Wei, Yangfei; Song, Zhibo; Li, Peipei; Li, Yulin] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Peoples R China; [Chen, Tao; Li, Hongmei; Shen, Cheng; Yan, Shuping; Wei, Yangfei; Song, Zhibo; Li, Peipei] Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Chen, Tao,Wang, Shuo,Li, Hongmei,et al. Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography[J]. 中国科学院西北生态环境资源研究院,2020.
APA	Chen, Tao.,Wang, Shuo.,Li, Hongmei.,Shen, Cheng.,Yan, Shuping.,...&Li, Yulin.(2020).Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography.JOURNAL OF CHROMATOGRAPHIC SCIENCE.
MLA	Chen, Tao,et al."Efficient One-Step Separation of Five Flavonoids from the Crude Extract of the Waste Pomace of Sea Buckthorn Berries through Counter-Current Chromatography".JOURNAL OF CHROMATOGRAPHIC SCIENCE (2020).