气候变化领域集成服务门户(CCPortal): Functional R2R3-MYB transcription factor NsMYB1, regulating anthocyanin biosynthesis, was relative to the fruit color differentiation in Nitraria sibirica Pall.

CCPortal

DOI	10.1186/s12870-022-03561-5
	Functional R2R3-MYB transcription factor NsMYB1, regulating anthocyanin biosynthesis, was relative to the fruit color differentiation in Nitraria sibirica Pall.
	Bao, Xuemei; Zong, Yuan; Hu, Na; Li, Shiming; Liu, Baolong; Wang, Honglun
通讯作者	Wang, HL (通讯作者)，Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Peoples R China. ; Wang, HL (通讯作者)，Northwest Inst Plateau Biol, CAS Key Lab Tibetan Med Res, Xining 810008, Peoples R China.
发表日期	2022
ISSN	1471-2229
卷号	22 期号:1
英文摘要	Background Nitraria sibirica Pall. is an economic plant with two kinds of fruit color, widely spreads in the Qinghai Tibet Plateau. The chemical analysis and pharmacological evaluation had been carried out for several tens of years, the mechanism behind the fruit color differentiation is still unclear. Results In this manuscript, the chemical analysis of the extractions showed that the chemical composition of fruit color was anthocyanin, and two kind of Nitraria sibirica Pall. were caused by the content differentiation with the same anthocyanin kinds. Cyanidin-3-[2-(6 '-coumaroyl)-glucosyl]-glucoside (C3G) was the major anthocyanin. Transcriptome analysis and the qRT-PCR revealed that the structural genes relative to anthocyanin biosynthesis except CHS, F3'5'H and ANS were up-regulated in the peels of BF (Black fruit) compared with the peels of RF (Red fruit), which indicated that transcript factor should be the reason for the expression difference of the structure genes. In the unigenes of the transcript factor MYB and bHLH, relative to anthocyanin, only NsMYB1 (Cluster 8422.10600), was high-expression and up-expression in the peels of BF. NsMYB1 encoded the same length protein with four amino acid differences in the RF and BF, and both contained the intact DNA, HTH-MYB and SANT domains. NsMYB1 was close to the AtMYB114, AtMYB113 and AtPAP1, regulating anthocyanin biosynthesis, in phylogenetic relationship. Both NsMYB1r and NsMYB1b could promote the transcript of the structural genes, and induced the anthocyanin accumulation in all tissues of transgenic tobacco. The insertion of 'TATA' in the promoter of NsMYB1r gave one more promoter region, and was the reason for higher transcripts in black fruit possibly. Conclusions Cyanidin-3-[2 ''-(6 '-coumaroyl)-glucosyl]-glucoside was the major anthocyanin in black fruit of Nitraria sibirica Pall.. NsMYB1 was a functional R2R3-MYB transcription factor, regulated the anthocyanin biosynthesis, and led to the fruit color differentiation in Nitraria sibirica Pall.
关键词	ANTIOXIDANT ACTIVITY RED COLORATION GENES PATHWAY
英文关键词	Nitraria sibirica Pall; Fruit color; Anthocyanin biosynthesis; MYB transcription factor; Tobacco
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000781383700004
来源期刊	BMC PLANT BIOLOGY
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253750
作者单位	[Bao, Xuemei; Hu, Na; Wang, Honglun] Northwest Inst Plateau Biol, Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Peoples R China; [Bao, Xuemei; Hu, Na; Wang, Honglun] Northwest Inst Plateau Biol, CAS Key Lab Tibetan Med Res, Xining 810008, Peoples R China; [Bao, Xuemei] Qinghai Normal Univ, Coll Educ, Xining 810008, Peoples R China; [Bao, Xuemei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zong, Yuan; Liu, Baolong] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota AEPB, Xining 810008, Peoples R China; [Li, Shiming] BGI Shenzhen, BGI Inst Appl Agr, Shenzhen 518120, Peoples R China
推荐引用方式 GB/T 7714	Bao, Xuemei,Zong, Yuan,Hu, Na,et al. Functional R2R3-MYB transcription factor NsMYB1, regulating anthocyanin biosynthesis, was relative to the fruit color differentiation in Nitraria sibirica Pall.[J]. 中国科学院西北生态环境资源研究院,2022,22(1).
APA	Bao, Xuemei,Zong, Yuan,Hu, Na,Li, Shiming,Liu, Baolong,&Wang, Honglun.(2022).Functional R2R3-MYB transcription factor NsMYB1, regulating anthocyanin biosynthesis, was relative to the fruit color differentiation in Nitraria sibirica Pall..BMC PLANT BIOLOGY,22(1).
MLA	Bao, Xuemei,et al."Functional R2R3-MYB transcription factor NsMYB1, regulating anthocyanin biosynthesis, was relative to the fruit color differentiation in Nitraria sibirica Pall.".BMC PLANT BIOLOGY 22.1(2022).