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DOI	10.1016/j.jchromb.2021.122981
	Comprehensive screening and separation of cyclooxygenase-2 inhibitors from Pterocephalus hookeri by affinity solid-phase extraction coupled with preparative high-performance liquid chromatography
	Zhu, Yunhe; Wang, Weidong; Liu, Zenggen; Jiang, Sirong; Tao, Yanduo; Jiang, Lei; Mei, Lijuan
通讯作者	Jiang, L ; Mei, LJ (通讯作者)，Chinese Acad Sci, Northwest Inst Plateau Biol, Northwest Inst Plateau Res, Key Lab Tibetan Med Res, Xining 810001, Peoples R China.
发表日期	2021
ISSN	1570-0232
EISSN	1873-376X
卷号	1183
英文摘要	Pterocephalus hookeri, a classical Tibetan herb, is mainly used to treat rheumatoid arthritis (RA) and contains various constituents potentially with cyclooxygenase-2 (COX-2) selective inhibition. A novel strategy for screening and target separating COX-2 inhibitors from the extracts of P. hookeri based on affinity solid-phase extraction (ASPE) column combined with preparative high-performance liquid chromatography (pre-HPLC) was successfully developed. The potential COX-2 inhibitors of P. hookeri were screened and recognized by the ASPE-HPLC system, which strategy is to analyze the compounds isolated by the ASPE column. Then, the active compounds were targeted separated by pre-HPLC according to real-time chromatograms. The control drugs celecoxib and glipizide were analyzed to verify the specificity and accuracy of the developed method. As a result, two pure compounds with COX-2 binding affinities were successfully separated from P. hookeri. They were characterized as swertisin and scopoletin using H-1- and C-13 NMR spectroscopy, and the in vitro COX-2 inhibitory activities were verified. Compounds with COX-2 inhibitory activities could be screened and targeted separated from crude extracts by this strategy, which indicated that the proposed method was feasible, robust and effective for rapid separation of COX-2 inhibitors from natural products.
关键词	XANTHINE-OXIDASE INHIBITORS BINDING ONLINE IDENTIFICATION COLUMN
英文关键词	Pterocephalus hookeri; Cyclooxygenase-2 inhibitors; Affinity solid-phase extraction; Preparative HPLC; Target separation
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000710587700006
来源期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/253731
作者单位	[Zhu, Yunhe; Wang, Weidong; Liu, Zenggen; Jiang, Sirong; Tao, Yanduo; Jiang, Lei; Mei, Lijuan] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 8100001, Peoples R China; [Zhu, Yunhe; Wang, Weidong; Liu, Zenggen; Jiang, Sirong; Tao, Yanduo; Jiang, Lei; Mei, Lijuan] Qinghai Prov Key Lab Tibetan Med Res, Xining 810001, Peoples R China; [Zhu, Yunhe; Wang, Weidong; Jiang, Sirong] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Yunhe,Wang, Weidong,Liu, Zenggen,et al. Comprehensive screening and separation of cyclooxygenase-2 inhibitors from Pterocephalus hookeri by affinity solid-phase extraction coupled with preparative high-performance liquid chromatography[J]. 中国科学院西北生态环境资源研究院,2021,1183.
APA	Zhu, Yunhe.,Wang, Weidong.,Liu, Zenggen.,Jiang, Sirong.,Tao, Yanduo.,...&Mei, Lijuan.(2021).Comprehensive screening and separation of cyclooxygenase-2 inhibitors from Pterocephalus hookeri by affinity solid-phase extraction coupled with preparative high-performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1183.
MLA	Zhu, Yunhe,et al."Comprehensive screening and separation of cyclooxygenase-2 inhibitors from Pterocephalus hookeri by affinity solid-phase extraction coupled with preparative high-performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1183(2021).