气候变化领域集成服务门户(CCPortal): A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis

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DOI	10.1073/pnas.2022583118
	A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis
	Mukherjee S.; Paricio N.; Sokol N.S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:21
英文摘要	Adult organisms must sense and adapt to environmental fluctuations. In high-turnover tissues such as the intestine, these adaptive responses require rapid changes in gene expression that, in turn, likely involve posttranscriptional gene control. However, intestinal-tissue–specific microRNA (miRNA)-mediated regulatory pathways remain unexplored. Here, we report the role of an intestinal-specific miRNA, miR-958, that non–cell autonomously regulates stem cell numbers during tissue homeostasis and regeneration in the Drosophila adult midgut. We identify its downstream target cabut, the Drosophila ortholog of mammalian KLF10/11 transcription factors, which mediates this miR-958 function by promoting paracrine enterocyte-to-stem-cell bone morphogenetic protein (BMP) signaling. We also show that mature miR-958 levels transiently decrease in response to stress and that this decrease is required for proper stem cell expansion during tissue regeneration. In summary, we have identified a posttranscriptional mechanism that modulates BMP signaling activity within Drosophila adult intestinal tissue during both normal homeostasis and tissue regeneration to regulate intestinal stem cell numbers. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	BMP signaling; Drosophila; Intestine; MicroRNA; Midgut
语种	英语
scopus关键词	bone morphogenetic protein; microRNA; microRNA 958; unclassified drug; bleomycin; bone morphogenetic protein; Cbt protein, Drosophila; Drosophila protein; green fluorescent protein; microRNA; MIRN958 microRNA, human; transcription factor; 3' untranslated region; adult; animal cell; animal experiment; animal tissue; Article; cell expansion; controlled study; down regulation; Drosophila; female; male; midgut; nonhuman; protein expression; signal transduction; tissue metabolism; tissue regeneration; upregulation; animal; cell count; cytology; Drosophila melanogaster; gene expression regulation; genetics; homeostasis; intestine cell; metabolism; regeneration; reporter gene; stem cell; Animals; Bleomycin; Bone Morphogenetic Proteins; Cell Count; Drosophila melanogaster; Drosophila Proteins; Enterocytes; Gene Expression Regulation; Genes, Reporter; Green Fluorescent Proteins; Homeostasis; MicroRNAs; Regeneration; Signal Transduction; Stem Cells; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251169
作者单位	Department of Biology, Indiana University, Bloomington, IN 47405, United States; Departamento de Genética, Facultad de Ciencies Biológicas and Estructura deRecerca Interdisciplinar en Biotecnologia i Biomedicina, University of Valencia, Burjasot, 46100, Spain
推荐引用方式 GB/T 7714	Mukherjee S.,Paricio N.,Sokol N.S.. A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis[J],2021,118(21).
APA	Mukherjee S.,Paricio N.,&Sokol N.S..(2021).A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis.Proceedings of the National Academy of Sciences of the United States of America,118(21).
MLA	Mukherjee S.,et al."A stress-responsive miRNA regulates BMP signaling to maintain tissue homeostasis".Proceedings of the National Academy of Sciences of the United States of America 118.21(2021).