气候变化领域集成服务门户(CCPortal): Spiny and soft-rayed fin domains in acanthomorph fish are established through a BMP-gremlin-shh signaling network

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DOI	10.1073/pnas.2101783118
	Spiny and soft-rayed fin domains in acanthomorph fish are established through a BMP-gremlin-shh signaling network
	Höch R.; Schneider R.F.; Kickuth A.; Meyer A.; Woltering J.M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:29
英文摘要	With over 18,000 species, the Acanthomorpha, or spiny-rayed fishes, form the largest and arguably most diverse radiation of vertebrates. One of the key novelties that contributed to their evolutionary success are the spiny rays in their fins that serve as a defense mechanism. We investigated the patterning mechanisms underlying the differentiation of median fin Anlagen into discrete spiny and soft-rayed domains during the ontogeny of the direct-developing cichlid fish Astatotilapia burtoni. Distinct transcription factor signatures characterize these two fin domains, whereby mutually exclusive expression of hoxa13a/b with alx4a/b and tbx2b marks the spine to soft-ray boundary. The soft-ray domain is established by BMP inhibition via gremlin1b, which synergizes in the posterior fin with shh secreted from a zone of polarizing activity. Modulation of BMP signaling by chemical inhibition or gremlin1b CRISPR/Cas9 knockout induces homeotic transformations of spines into soft rays and vice versa. The expression of spine and soft-ray genes in nonacanthomorph fins indicates that a combination of exaptation and posterior expansion of an ancestral developmental program for the anterior fin margin allowed the evolution of robustly individuated spiny and soft-rayed domains. We propose that a repeated exaptation of such pattern might underly the convergent evolution of anterior spiny-fin elements across fishes. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Acanthomorph; Evo-devo; Evolutionary key innovation; Exaptation; Fin spine
语种	英语
scopus关键词	cyclopamine; guide RNA; sonic hedgehog protein; bone morphogenetic protein; fish protein; signal peptide; sonic hedgehog protein; transcription factor; alx4 gene; alx4a gene; animal experiment; animal husbandry; animal model; Article; BMP signaling; catfish; cloning; controlled study; convergent evolution; CRISPR-CAS9 system; embryo; endoderm; fin (organ); fish; fluorescence microscopy; gene; gene expression; gene sequence; genotype; gremlin gene; high throughput sequencing; homeobox; hoxa13a gene; hoxa13b gene; in situ hybridization; knockout gene; male; nonhuman; phenotype; protein expression; RNA sequence; sequence analysis; shh gene; spine; tbx2b gene; zebra fish; animal; body patterning; cichlid; classification; evolution; fin (organ); gene expression regulation; genetics; growth, development and aging; metabolism; phylogeny; signal transduction; spine; Animal Fins; Animals; Biological Evolution; Body Patterning; Bone Morphogenetic Proteins; Cichlids; Fish Proteins; Gene Expression Regulation, Developmental; Hedgehog Proteins; Intercellular Signaling Peptides and Proteins; Phylogeny; Signal Transduction; Spine; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251122
作者单位	Zoology and Evolutionary Biology, Department of Biology, University of Konstanz, Konstanz, 78457, Germany
推荐引用方式 GB/T 7714	Höch R.,Schneider R.F.,Kickuth A.,et al. Spiny and soft-rayed fin domains in acanthomorph fish are established through a BMP-gremlin-shh signaling network[J],2021,118(29).
APA	Höch R.,Schneider R.F.,Kickuth A.,Meyer A.,&Woltering J.M..(2021).Spiny and soft-rayed fin domains in acanthomorph fish are established through a BMP-gremlin-shh signaling network.Proceedings of the National Academy of Sciences of the United States of America,118(29).
MLA	Höch R.,et al."Spiny and soft-rayed fin domains in acanthomorph fish are established through a BMP-gremlin-shh signaling network".Proceedings of the National Academy of Sciences of the United States of America 118.29(2021).