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DOI	10.1073/pnas.2100709118
	The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice
	Zheng K.; Wang L.; Zeng L.; Xu D.; Guo Z.; Gao X.; Yang D.-L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:30
英文摘要	RNA-directed DNA methylation (RdDM) functions in de novo methylation in CG, CHG, and CHH contexts. Here, we performed map-based cloning of OsNRPE1, which encodes the largest subunit of RNA polymerase V (Pol V), a key regulator of gene silencing and reproductive development in rice. We found that rice Pol V is required for CHH methylation on RdDM loci by transcribing long noncoding RNAs. Pol V influences the accumulation of 24-nucleotide small interfering RNAs (24-nt siRNAs) in a locus-specific manner. Bio-synthesis of 24-nt siRNAs on loci with high CHH methylation levels and low CG and CHG methylation levels tends to depend on Pol V. In contrast, low methylation levels in the CHH context and high methylation levels in CG and CHG contexts predisposes 24-nt siRNA accumulation to be independent of Pol V. H3K9me1 and H3K9me2 tend to be enriched on Pol V–independent 24-nt siRNA loci, whereas various active histone modifications are enriched on Pol V–dependent 24-nt siRNA loci. DNA methylation is required for 24-nt siRNAs biosynthesis on Pol V–dependent loci but not on Pol V–independent loci. Our results reveal the function of rice Pol V for long noncoding RNA production, DNA methylation, 24-nt siRNA accumulation, and reproductive development. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	OsNRPE1; Rice; RNA polymerase V; siRNA
语种	英语
scopus关键词	24 nucleotide small interfering RNA; long untranslated RNA; RNA polymerase; RNA polymerase V; small interfering RNA; unclassified drug; DNA directed RNA polymerase; plant protein; plant RNA; small interfering RNA; agronomic trait; Article; Chop PCR assay; controlled study; CRISPR-CAS9 system; DNA methylation; gene locus; gene silencing; histone modification; molecular cloning; nonhuman; OsNRPE1 gene; polymerase chain reaction; promoter region; rice; RNA sequencing; RNA synthesis; small RNA sequencing; transgene; whole genome bisulfite sequencing; gene expression regulation; genetics; growth, development and aging; histone code; metabolism; Oryza; DNA Methylation; DNA-Directed RNA Polymerases; Gene Expression Regulation, Plant; Gene Silencing; Histone Code; Oryza; Plant Proteins; RNA, Plant; RNA, Small Interfering
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251110
作者单位	State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China; Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, 210095, China; Vector-borne Virus Research Center, Fujian Agriculture and Forestry University, Fujian, 350002, China
推荐引用方式 GB/T 7714	Zheng K.,Wang L.,Zeng L.,et al. The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice[J],2021,118(30).
APA	Zheng K..,Wang L..,Zeng L..,Xu D..,Guo Z..,...&Yang D.-L..(2021).The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice.Proceedings of the National Academy of Sciences of the United States of America,118(30).
MLA	Zheng K.,et al."The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice".Proceedings of the National Academy of Sciences of the United States of America 118.30(2021).