气候变化领域集成服务门户(CCPortal): Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2

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DOI	10.1073/pnas.2100073118
	Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2
	Kamiyama Y.; Hirotani M.; Ishikawa S.; Minegishi F.; Katagiri S.; Rogan C.J.; Takahashi F.; Nomoto M.; Ishikawa K.; Kodama Y.; Tada Y.; Takezawa D.; Anderson J.C.; Peck S.C.; Shinozaki K.; Umezawa T.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:30
英文摘要	The phytohormone abscisic acid (ABA) plays a major role in abiotic stress responses in plants, and subclass III SNF1-related protein kinase 2 (SnRK2) kinases mediate ABA signaling. In this study, we identified Raf36, a group C Raf-like protein kinase in Arabidopsis, as a protein that interacts with multiple SnRK2s. A series of reverse genetic and biochemical analyses revealed that 1) Raf36 negatively regulates ABA responses during postgermination growth, 2) the N terminus of Raf36 is directly phosphorylated by SnRK2s, and 3) Raf36 degradation is enhanced in response to ABA. In addition, Raf22, another C-type Raf-like kinase, functions partially redundantly with Raf36 to regulate ABA responses. A comparative phosphoproteomic analysis of ABA-induced responses of wild-type and raf22raf36-1 plants identified proteins that are phosphorylated downstream of Raf36 and Raf22 in planta. Together, these results support a model in which Raf36/Raf22 function mainly under optimal conditions to suppress ABA responses, whereas in response to ABA, the SnRK2 module promotes Raf36 degradation as a means of alleviating Raf36-dependent inhibition and allowing for heightened ABA signaling to occur. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Abscisic acid; Arabidopsis thaliana; Raf-like kinase; SnRK2
语种	英语
scopus关键词	abscisic acid; green fluorescent protein; maltose binding protein; protein kinase; Raf protein; SNF1 related protein kinase 2; unclassified drug; abscisic acid; Arabidopsis protein; phytohormone; SnRK2 protein, Arabidopsis; abiotic stress; amino terminal sequence; Arabidopsis; Arabidopsis thaliana; Article; autophosphorylation; biochemical analysis; chloroplast; enzyme activity; fungus growth; gene expression; immune response; immunoblotting; immunoprecipitation; liquid chromatography-mass spectrometry; mass spectrometry; Nicotiana benthamiana; nonhuman; phosphoproteomics; plant growth; protein degradation; protein phosphorylation; protein protein interaction; protein synthesis; RNA extraction; validation process; Arabidopsis; drug effect; gene expression regulation; genetics; growth, development and aging; metabolism; phosphorylation; physiological stress; signal transduction; Abscisic Acid; Arabidopsis; Arabidopsis Proteins; Gene Expression Regulation, Plant; Phosphorylation; Plant Growth Regulators; Protein Serine-Threonine Kinases; Signal Transduction; Stress, Physiological
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251108
作者单位	Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Tokyo, 184-8588, Japan; Department ofBotany and Plant Pathology, Oregon State University, Corvallis, OR 97331, United States; Gene Discovery Research Group, RIKEN Center for Sustainable Resource Science, Ibaraki305-0074, Japan; Division of Biological Science, Nagoya University, Aichi, 464-8602, Japan; Center for Gene Research, Nagoya University, Aichi, 464-8602, Japan; Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505, Japan; Graduate School of Science, Engineering, Saitama University, Saitama, 338-8570, Japan; Department of Biochemistry, University of Missouri, Columbia, MO 65211, United States; Faculty ofAgriculture, Tokyo University of Agriculture and Technology, Tokyo, 183-8538, Japan; PRESTO, Japan Science and Technology Agency, Saitama, 332-0012, Japan
推荐引用方式 GB/T 7714	Kamiyama Y.,Hirotani M.,Ishikawa S.,et al. Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2[J],2021,118(30).
APA	Kamiyama Y..,Hirotani M..,Ishikawa S..,Minegishi F..,Katagiri S..,...&Umezawa T..(2021).Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2.Proceedings of the National Academy of Sciences of the United States of America,118(30).
MLA	Kamiyama Y.,et al."Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2".Proceedings of the National Academy of Sciences of the United States of America 118.30(2021).