气候变化领域集成服务门户(CCPortal): Three mutations repurpose a plant karrikin receptor to a strigolactone receptor

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DOI	10.1073/pnas.2103175118
	Three mutations repurpose a plant karrikin receptor to a strigolactone receptor
	Arellano-Saab A.; Bunsick M.; Galib H.A.; Zhao W.; Schuetz S.; Bradley J.M.; Xu Z.; Adityani C.; Subha A.; McKay H.; de Saint Germain A.; Boyer F.-D.; McErlean C.S.P.; Toh S.; McCourt P.; Stogios P.J.; Lumba S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:30
英文摘要	Uncovering the basis of small-molecule hormone receptors’ evolution is paramount to a complete understanding of how protein structure drives function. In plants, hormone receptors for strigolactones are well suited to evolutionary inquiries because closely related homologs have different ligand preferences. More importantly, because of facile plant transgenic systems, receptors can be swapped and quickly assessed functionally in vivo. Here, we show that only three mutations are required to turn the nonstrigolactone receptor, KAI2, into a receptor that recognizes the plant hormone strigolactone. This modified receptor still retains its native function to perceive KAI2 ligands. Our directed evolution studies indicate that only a few keystone mutations are required to increase receptor promiscuity of KAI2, which may have implications for strigolactone receptor evolution in parasitic plants. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Karrikins; Plant hormone; Plants; Receptor evolution; Strigolactones
语种	英语
scopus关键词	KAI2 receptor; karrikin receptor; ligand; phytohormone; plant protein; receptor; strigolactone; strigolactone receptor; unclassified drug; 3-methyl-2H-furo(2,3-c)pyran-2-one; Arabidopsis protein; furan derivative; fused heterocyclic rings; GR24 strigolactone; HTL protein, Arabidopsis; hydrolase; lactone; phytohormone; protein binding; pyran derivative; Article; controlled study; gene mutation; molecular evolution; mutational analysis; nonhuman; parasitic plant; protein function; Arabidopsis; gene expression regulation; genetics; metabolism; mutation; phylogeny; physiology; Arabidopsis; Arabidopsis Proteins; Furans; Gene Expression Regulation, Plant; Heterocyclic Compounds, 3-Ring; Hydrolases; Lactones; Mutation; Phylogeny; Plant Growth Regulators; Protein Binding; Pyrans
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251104
作者单位	Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 3E5, Canada; Institut Jean-Pierre Bourgin, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement, AgroParisTech, Université Paris-Saclay, Versailles, 78000, France; Institut de Chimie des Substances Naturelles, CNRS, Université Paris-Saclay, Gif-sur-Yvette, 91198, France; School of Chemistry, University of Sydney, Sydney, NSW 2006, Australia; Department of Environmental Bioscience, School of Agriculture, Meijo University, Nagoya, 468-8502, Japan
推荐引用方式 GB/T 7714	Arellano-Saab A.,Bunsick M.,Galib H.A.,et al. Three mutations repurpose a plant karrikin receptor to a strigolactone receptor[J],2021,118(30).
APA	Arellano-Saab A..,Bunsick M..,Galib H.A..,Zhao W..,Schuetz S..,...&Lumba S..(2021).Three mutations repurpose a plant karrikin receptor to a strigolactone receptor.Proceedings of the National Academy of Sciences of the United States of America,118(30).
MLA	Arellano-Saab A.,et al."Three mutations repurpose a plant karrikin receptor to a strigolactone receptor".Proceedings of the National Academy of Sciences of the United States of America 118.30(2021).