气候变化领域集成服务门户(CCPortal): Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors

CCPortal

DOI	10.1073/pnas.2103027118
	Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors
	Hockenberry A.M.; Micali G.; Takács G.; Weng J.; Hardt W.-D.; Ackermann M.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:31
英文摘要	Salmonella spp. express Salmonella pathogenicity island 1 Type III Secretion System 1 (T3SS-1) genes to mediate the initial phase of interaction with their host. Prior studies indicate short-chain fatty acids, microbial metabolites at high concentrations in the gastrointestinal tract, limit population-level T3SS-1 gene expression. However, only a subset of Salmonella cells in a population express these genes, suggesting short-chain fatty acids could decrease T3SS-1 population-level expression by acting on per-cell expression or the proportion of expressing cells. Here, we combine single-cell, theoretical, and molecular approaches to address the effect of short-chain fatty acids on T3SS-1 expression. Our in vitro results show short-chain fatty acids do not repress T3SS-1 expression by individual cells. Rather, these compounds act to selectively slow the growth of T3SS-1-expressing cells, ultimately decreasing their frequency in the population. Further experiments indicate slowed growth arises from short-chain fatty acid-mediated depletion of the proton motive force. By influencing the T3SS-1 cell-type proportions, our findings imply gut microbial metabolites act on cooperation between the two cell types and ultimately influence Salmonella's capacity to establish within a host. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Pathogenesis; Salmonella; Single cell
语种	英语
scopus关键词	bacterial protein; Spi1 protein, Salmonella; volatile fatty acid; culture medium; drug effect; gene expression regulation; genetics; metabolism; microbiological examination; microfluidics; Salmonella; Bacterial Proteins; Bacteriological Techniques; Culture Media; Fatty Acids, Volatile; Gene Expression Regulation, Bacterial; Microfluidics; Salmonella
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251086
作者单位	Department of Environmental Systems Science, ETH Zürich, Zürich, 8092, Switzerland; Department of Environmental Microbiology, Eawag, Dübendorf, 8600, Switzerland; Institute of Microbiology, Department of Biology, ETH Zürich, Zürich, 8093, Switzerland; Mayo Clinic Alix School of Medicine, Mayo Clinic College of Medicine and Science, Mayo Clinic, Rochester, MN 55905, United States
推荐引用方式 GB/T 7714	Hockenberry A.M.,Micali G.,Takács G.,et al. Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors[J],2021,118(31).
APA	Hockenberry A.M.,Micali G.,Takács G.,Weng J.,Hardt W.-D.,&Ackermann M..(2021).Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors.Proceedings of the National Academy of Sciences of the United States of America,118(31).
MLA	Hockenberry A.M.,et al."Microbiota-derived metabolites inhibit Salmonella virulent subpopulation development by acting on single-cell behaviors".Proceedings of the National Academy of Sciences of the United States of America 118.31(2021).