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DOI	10.1073/pnas.2026719118
	Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope
	Pérez-Ruiz M.; Pulido-Cid M.; Luque-Ortega J.R.; Valpuesta J.M.; Cuervo A.; Carrascosa J.L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:34
英文摘要	In most bacteriophages, genome transport across bacterial envelopes is carried out by the tail machinery. In viruses of the Podoviridae family, in which the tail is not long enough to traverse the bacterial wall, it has been postulated that viral core proteins assembled inside the viral head are translocated and reassembled into a tube within the periplasm that extends the tail channel. Bacteriophage T7 infects Escherichia coli, and despite extensive studies, the precise mechanism by which its genome is translocated remains unknown. Using cryo-electron microscopy, we have resolved the structure of two different assemblies of the T7 DNA translocation complex composed of the core proteins gp15 and gp16. Gp15 alone forms a partially folded hexamer, which is further assembled upon interaction with gp16 into a tubular structure, forming a channel that could allow DNA passage. The structure of the gp15–gp16 complex also shows the location within gp16 of a canonical transglycosylase motif involved in the degradation of the bacterial peptidoglycan layer. This complex docks well in the tail extension structure found in the periplasm of T7-infected bacteria and matches the sixfold symmetry of the phage tail. In such cases, gp15 and gp16 that are initially present in the T7 capsid eightfold-symmetric core would change their oligomeric state upon reassembly in the periplasm. Altogether, these results allow us to propose a model for the assembly of the core translocation complex in the periplasm, which furthers understanding of the molecular mechanism involved in the release of T7 viral DNA into the bacterial cytoplasm. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacteriophage; cryo-EM; DNA translocation; Protein structure; Transglycosylase
语种	英语
scopus关键词	core protein; morpholino oligonucleotide; virus DNA; amino acid sequence; cryoelectron microscopy; electron microscopy; Enterobacteria phage T7; gene expression regulation; gene translocation; genetics; image processing; metabolism; molecular model; physiology; protein conformation; virus entry; Amino Acid Sequence; Bacteriophage T7; Cryoelectron Microscopy; DNA, Viral; Gene Expression Regulation, Viral; Image Processing, Computer-Assisted; Microscopy, Electron; Models, Molecular; Morpholinos; Protein Conformation; Translocation, Genetic; Viral Core Proteins; Virus Internalization
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/251048
作者单位	Centro Nacional de Biotecnología, Madrid, 28049, Spain; Centro de Investigaciones Biológicas Margarita Salas, Madrid, 28040, Spain
推荐引用方式 GB/T 7714	Pérez-Ruiz M.,Pulido-Cid M.,Luque-Ortega J.R.,et al. Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope[J],2021,118(34).
APA	Pérez-Ruiz M.,Pulido-Cid M.,Luque-Ortega J.R.,Valpuesta J.M.,Cuervo A.,&Carrascosa J.L..(2021).Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope.Proceedings of the National Academy of Sciences of the United States of America,118(34).
MLA	Pérez-Ruiz M.,et al."Assisted assembly of bacteriophage T7 core components for genome translocation across the bacterial envelope".Proceedings of the National Academy of Sciences of the United States of America 118.34(2021).