气候变化领域集成服务门户(CCPortal): Cell density, alignment, and orientation correlate with C-signal-dependent gene expression during Myxococcus xanthus development

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DOI	10.1073/pnas.2111706118
	Cell density, alignment, and orientation correlate with C-signal-dependent gene expression during Myxococcus xanthus development
	Hoang Y.; Franklin J.L.; Dufour Y.S.; Kroos L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:45
英文摘要	Starving Myxococcus xanthus bacteria use short-range C-signaling to coordinate their movements and construct multicellular mounds, which mature into fruiting bodies as rods differentiate into spherical spores. Differentiation requires efficient C-signaling to drive the expression of developmental genes, but how the arrangement of cells within nascent fruiting bodies (NFBs) affects C-signaling is not fully understood. Here, we used confocal microscopy and cell segmentation to visualize and quantify the arrangement, morphology, and gene expression of cells near the bottom of NFBs at much higher resolution than previously achieved. We discovered that "transitioning cells" (TCs), intermediate in morphology between rods and spores, comprised 10 to 15% of the total population. Spores appeared midway between the center and the edge of NFBs early in their development and near the center as maturation progressed. The developmental pattern, as well as C-signal-dependent gene expression in TCs and spores, were correlated with cell density, the alignment of neighboring rods, and the tangential orientation of rods early in the development of NFBs. These dynamic radial patterns support a model in which the arrangement of cells within the NFBs affects C-signaling efficiency to regulate precisely the expression of developmental genes and cellular differentiation in space and time. Developmental patterns in other bacterial biofilms may likewise rely on short-range signaling to communicate multiple aspects of cellular arrangement, analogous to juxtacrine and paracrine signaling during animal development. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacterial development; Biofilm; Cell-cell signaling; Pattern formation; Spore differentiation
语种	英语
scopus关键词	article; bacterial growth; bacterial spore; biofilm; body patterning; cell density; cell differentiation; confocal microscopy; controlled study; developmental gene; fruiting body; gene expression; human; Myxococcus xanthus; nonhuman; paracrine signaling; protein expression; bacterial spore; cytology; gene expression regulation; Myxococcus xanthus; organismal interaction; physiology; Gene Expression Regulation, Bacterial; Microbial Interactions; Myxococcus xanthus; Spores, Bacterial
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250997
作者单位	Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, United States; Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, United States
推荐引用方式 GB/T 7714	Hoang Y.,Franklin J.L.,Dufour Y.S.,et al. Cell density, alignment, and orientation correlate with C-signal-dependent gene expression during Myxococcus xanthus development[J],2021,118(45).
APA	Hoang Y.,Franklin J.L.,Dufour Y.S.,&Kroos L..(2021).Cell density, alignment, and orientation correlate with C-signal-dependent gene expression during Myxococcus xanthus development.Proceedings of the National Academy of Sciences of the United States of America,118(45).
MLA	Hoang Y.,et al."Cell density, alignment, and orientation correlate with C-signal-dependent gene expression during Myxococcus xanthus development".Proceedings of the National Academy of Sciences of the United States of America 118.45(2021).