气候变化领域集成服务门户(CCPortal): Biological signal processing filters via engineering allosteric transcription factors

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DOI	10.1073/pnas.2111450118
	Biological signal processing filters via engineering allosteric transcription factors
	Groseclose T.M.; Hersey A.N.; Huang B.D.; Realff M.J.; Wilson C.J.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:46
英文摘要	Signal processing is critical to a myriad of biological phenomena (natural and engineered) that involve gene regulation. Biological signal processing can be achieved by way of allosteric transcription factors. In canonical regulatory systems (e.g., the lactose repressor), an INPUT signal results in the induction of a given transcription factor and objectively switches gene expression from an OFF state to an ON state. In such biological systems, to revert the gene expression back to the OFF state requires the aggressive dilution of the input signal, which can take 1 or more d to achieve in a typical biotic system. In this study, we present a class of engineered allosteric transcription factors capable of processing two-signal INPUTS, such that a sequence of INPUTS can rapidly transition gene expression between alternating OFF and ON states. Here, we present two fundamental biological signal processing filters, BANDPASS and BANDSTOP, that are regulated by D-fucose and isopropyl- β-D-1-thiogalactopyranoside. BANDPASS signal processing filters facilitate OFF-ON-OFF gene regulation.Whereas, BANDSTOP filters facilitate the antithetical gene regulation, ON-OFF-ON. Engineered signal processing filters can be directed to seven orthogonal promoters via adaptive modular DNA binding design. This collection of signal processing filters can be used in collaboration with our established transcriptional programming structure. Kinetic studies show that our collection of signal processing filters can switch between states of gene expression within a few minutes with minimal metabolic burden-representing a paradigm shift in general gene regulation. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	BANDPASS; BANDSTOP; Protein engineering; Synthetic biology; Transcription factors
语种	英语
scopus关键词	fucose; isopropyl thiogalactoside; transcription factor; protein binding; transcription factor; allosterism; Article; BANDPASS signal processing filter; BANDSTOP signal processing filter; biological signal processing filter; controlled study; DNA binding; gene control; gene expression; promoter region; protein engineering; signal transduction; synthetic biology; allosterism; devices; Escherichia coli; gene expression regulation; gene regulatory network; genetics; kinetics; procedures; signal processing; Allosteric Regulation; Escherichia coli; Gene Expression; Gene Expression Regulation; Gene Regulatory Networks; Kinetics; Promoter Regions, Genetic; Protein Binding; Protein Engineering; Signal Processing, Computer-Assisted; Synthetic Biology; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250991
作者单位	School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA 30332-2000, United States
推荐引用方式 GB/T 7714	Groseclose T.M.,Hersey A.N.,Huang B.D.,et al. Biological signal processing filters via engineering allosteric transcription factors[J],2021,118(46).
APA	Groseclose T.M.,Hersey A.N.,Huang B.D.,Realff M.J.,&Wilson C.J..(2021).Biological signal processing filters via engineering allosteric transcription factors.Proceedings of the National Academy of Sciences of the United States of America,118(46).
MLA	Groseclose T.M.,et al."Biological signal processing filters via engineering allosteric transcription factors".Proceedings of the National Academy of Sciences of the United States of America 118.46(2021).