气候变化领域集成服务门户(CCPortal): Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling

CCPortal

DOI	10.1073/pnas.2115292118
	Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling
	Barnett S.E.; Youngblut N.D.; Koechli C.N.; Buckley D.H.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:47
英文摘要	Soil microorganisms determine the fate of soil organic matter (SOM), and their activities compose a major component of the global carbon (C) cycle. We employed a multisubstrate, DNA-stable isotope probing experiment to track bacterial assimilation of C derived from distinct sources that varied in bioavailability. This approach allowed us to measure microbial contributions to SOM processing by measuring the C assimilation dynamics of diverse microorganisms as they interacted within soil. We identified and tracked 1,286 bacterial taxa that assimilated 13C in an agricultural soil over a period of 48 d. Overall 13C-assimilation dynamics of bacterial taxa, defined by the source and timing of the 13C they assimilated, exhibited low phylogenetic conservation. We identified bacterial guilds composed of taxa that had similar 13C assimilation dynamics. We show that C-source bioavailability explained significant variation in both C mineralization dynamics and guild structure, and that the growth dynamics of bacterial guilds differed significantly in response to C addition. We also demonstrate that the guild structure explains significant variation in the biogeographical distribution of bacteria at continental and global scales. These results suggest that an understanding of in situ growth dynamics is essential for understanding microbial contributions to soil C cycling.We interpret these findings in the context of bacterial life history strategies and their relationship to terrestrial C cycling. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacterial; C cycle; DNA-SIP; Ecology; Soil
语种	英语
scopus关键词	bacterial DNA; carbon 13; soil organic matter; carbon; DNA; isotope; Article; bacterial genetics; bacterial growth; bacterial structures; bacterium identification; bioavailability; biogeographic region; carbon cycling; carbon fixation; carbon mineralization; carbon source; microbial interaction; nonhuman; phylogeny; soil microflora; agriculture; bacterium; carbon cycle; chemistry; genetics; isotope labeling; microbiology; procedures; soil; Agriculture; Bacteria; Carbon; Carbon Cycle; DNA; Isotope Labeling; Isotopes; Phylogeny; Soil; Soil Microbiology
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250977
作者单位	School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, United States; Department of Microbiome Science, Max Planck Institute for Developmental Biology, Tubingen, 72076, Germany; Department of Biological Sciences, University of the Sciences, Philadelphia, PA 19104, United States
推荐引用方式 GB/T 7714	Barnett S.E.,Youngblut N.D.,Koechli C.N.,et al. Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling[J],2021,118(47).
APA	Barnett S.E.,Youngblut N.D.,Koechli C.N.,&Buckley D.H..(2021).Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling.Proceedings of the National Academy of Sciences of the United States of America,118(47).
MLA	Barnett S.E.,et al."Multisubstrate DNA stable isotope probing reveals guild structure of bacteria that mediate soil carbon cycling".Proceedings of the National Academy of Sciences of the United States of America 118.47(2021).