气候变化领域集成服务门户(CCPortal): Molecular mechanisms of sperm motility are conserved in an early-branching metazoan

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DOI	10.1073/pnas.2109993118
	Molecular mechanisms of sperm motility are conserved in an early-branching metazoan
	Speer K.F.; Allen-Waller L.; Novikov D.R.; Barott K.L.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:48
英文摘要	Efficient and targeted sperm motility is essential for animal reproductive success. Sperm from mammals and echinoderms utilize a highly conserved signaling mechanism in which sperm motility is stimulated by pH-dependent activation of the cAMP-producing enzyme soluble adenylyl cyclase (sAC). However, the presence of this pathway in early-branching metazoans has remained unexplored. Here, we found that elevating cytoplasmic pH induced a rapid burst of cAMP signaling and triggered the onset of motility in sperm from the reef-building coral Montipora capitata in a sAC-dependent manner. Expression of sAC in the mitochondrial-rich midpiece and flagellum of coral sperm support a dual role for this molecular pH sensor in regulating mitochondrial respiration and flagellar beating and thus motility. In addition, we found that additional members of the homologous signaling pathway described in echinoderms, both upstream and downstream of sAC, are expressed in coral sperm. These include the Na+/H+ exchanger SLC9C1, protein kinase A, and the CatSper Ca2+ channel conserved even in mammalian sperm. Indeed, the onset of motility corresponded with increased protein kinase A activity. Our discovery of this pathway in an early-branching metazoan species highlights the ancient origin of the pH-sAC-cAMP signaling node in sperm physiology and suggests that it may be present in many other marine invertebrate taxa for which sperm motility mechanisms remain unexplored. These results emphasize the need to better understand the role of pH-dependent signaling in the reproductive success of marine animals, particularly as climate change stressors continue to alter the physiology of corals and other marine invertebrates. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Coral; Cyclic AMP; PH; Reproduction; Soluble adenylyl cyclase
语种	英语
scopus关键词	adenylate cyclase; calcium channel; cyclic AMP; cyclic AMP dependent protein kinase; sodium proton exchange protein; adenylate cyclase; calcium; cyclic AMP; cyclic AMP dependent protein kinase; adult; animal cell; animal tissue; Article; cAMP signaling; cell pH; climate change; controlled study; coral; echinoderm; enzyme activity; flagellum; mitochondrial respiration; Montipora capitata; nonhuman; protein expression; reproductive success; semen pH; spermatozoon midpiece; spermatozoon motility; animal; Anthozoa; biodiversity; homeostasis; male; metabolism; pH; phosphorylation; phylogeny; physiology; reproduction; spermatozoon; spermatozoon motility; Adenylyl Cyclases; Animals; Anthozoa; Biodiversity; Calcium; Cyclic AMP; Cyclic AMP-Dependent Protein Kinases; Flagella; Homeostasis; Hydrogen-Ion Concentration; Male; Phosphorylation; Phylogeny; Reproduction; Sperm Motility; Spermatozoa
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250964
作者单位	Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, United States
推荐引用方式 GB/T 7714	Speer K.F.,Allen-Waller L.,Novikov D.R.,et al. Molecular mechanisms of sperm motility are conserved in an early-branching metazoan[J],2021,118(48).
APA	Speer K.F.,Allen-Waller L.,Novikov D.R.,&Barott K.L..(2021).Molecular mechanisms of sperm motility are conserved in an early-branching metazoan.Proceedings of the National Academy of Sciences of the United States of America,118(48).
MLA	Speer K.F.,et al."Molecular mechanisms of sperm motility are conserved in an early-branching metazoan".Proceedings of the National Academy of Sciences of the United States of America 118.48(2021).