气候变化领域集成服务门户(CCPortal): Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots

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DOI	10.1073/pnas.2111521118
	Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots
	Wolinska K.W.; Vannier N.; Thiergart T.; Pickel B.; Gremmen S.; Piasecka A.; Piślewska-Bednarek M.; Nakano R.T.; Belkhadir Y.; Bednarek P.; Hacquard S.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:49
英文摘要	In nature, roots of healthy plants are colonized by multikingdom microbial communities that include bacteria, fungi, and oomycetes. A key question is how plants control the assembly of these diverse microbes in roots to maintain host–microbe homeostasis and health. Using microbiota reconstitution experiments with a set of immunocompromised Arabidopsis thaliana mutants and a multikingdom synthetic microbial community (SynCom) representative of the natural A. thaliana root microbiota, we observed that microbiota-mediated plant growth promotion was abolished in most of the tested immunocompromised mutants. Notably, more than 40% of between-genotype variation in these microbiota-induced growth differences was explained by fungal but not bacterial or oomycete load in roots. Extensive fungal overgrowth in roots and altered plant growth was evident at both vegetative and reproductive stages for a mutant impaired in the production of tryptophan-derived, specialized metabolites (cyp79b2/b3). Microbiota manipulation experiments with single- and multikingdom microbial SynComs further demonstrated that 1) the presence of fungi in the multikingdom SynCom was the direct cause of the dysbiotic phenotype in the cyp79b2/b3 mutant and 2) bacterial commensals and host tryptophan metabolism are both necessary to control fungal load, thereby promoting A. thaliana growth and survival. Our results indicate that protective activities of bacterial root commensals are as critical as the host tryptophan metabolic pathway in preventing fungal dysbiosis in the A. thaliana root endosphere. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Microbial homeostasis; Microbial interactions; Plant holobiont; Plant innate immunity; Root microbiome
语种	英语
scopus关键词	camalexin; indol 3 acetaldoxime; indol 3 carboxylic acid; indole derivative; indole glucosinolate; indoleacetic acid; unclassified drug; Arabidopsis protein; mycosis; tryptophan; Arabidopsis thaliana; Article; bacterial load; bacterium; biodiversity; biomanipulation; commensal; community structure; controlled study; cyp79b2 gene; cyp79b3 gene; developmental stage; fresh weight; fungal dysbiosis; fungal load; fungal phenomena and functions; fungus growth; genetic variability; growth inhibition; homeostasis; host microbe homeostasis; innate immunity; microbial community; microbial pest control; microflora; nonhuman; Oomycetes; pathogen load; phenotype; plant gene; plant growth; plant immunity; plant microorganism interaction; plant root; tryptophan metabolism; vegetative stage; Arabidopsis; bacterium; dysbiosis; fungus; genetics; growth, development and aging; metabolism; microbiology; physiology; plant development; plant root; symbiosis; Arabidopsis; Arabidopsis Proteins; Bacteria; Dysbiosis; Fungi; Microbiota; Mycoses; Oomycetes; Plant Development; Plant Roots; Soil Microbiology; Symbiosis; Tryptophan
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250952
作者单位	Department of Plant Microbe Interactions, Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany; Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, 61-704, Poland; Gregor Mendel Institute, Austrian Academy of Sciences, Vienna Biocenter, Vienna, 1030, Austria; Cluster of Excellence on Plant Sciences, Max Planck Institute for Plant Breeding Research, Cologne, 50829, Germany
推荐引用方式 GB/T 7714	Wolinska K.W.,Vannier N.,Thiergart T.,et al. Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots[J],2021,118(49).
APA	Wolinska K.W..,Vannier N..,Thiergart T..,Pickel B..,Gremmen S..,...&Hacquard S..(2021).Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots.Proceedings of the National Academy of Sciences of the United States of America,118(49).
MLA	Wolinska K.W.,et al."Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots".Proceedings of the National Academy of Sciences of the United States of America 118.49(2021).