气候变化领域集成服务门户(CCPortal): Bacteriophage self-counting in the presence of viral replication

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DOI	10.1073/pnas.2104163118
	Bacteriophage self-counting in the presence of viral replication
	Yao T.; Coleman S.; Nguyen T.V.P.; Golding I.; Igoshin O.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:51
英文摘要	When host cells are in low abundance, temperate bacteriophages opt for dormant (lysogenic) infection. Phage lambda implements this strategy by increasing the frequency of lysogeny at higher multiplicity of infection (MOI). However, it remains unclear how the phage reliably counts infecting viral genomes even as their intracellular number increases because of replication. By combining theoretical modeling with single-cell measurements of viral copy number and gene expression, we find that instead of hindering lambda’s decision, replication facilitates it. In a nonreplicating mutant, viral gene expression simply scales with MOI rather than diverging into lytic (virulent) and lysogenic trajectories. A similar pattern is followed during early infection by wild-type phage. However, later in the infection, the modulation of viral replication by the decision genes amplifies the initially modest gene expression differences into divergent trajectories. Replication thus ensures the optimal decision—lysis upon single-phage infection and lysogeny at higher MOI. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Bacteriophage; Cell-fate decision; E. coli; Mathematical modeling; Single cell
语种	英语
scopus关键词	article; bacteriophage; cell fate; controlled study; cytometry; Escherichia coli; lysogenization; nonhuman; virus replication; wild type; biological model; Enterobacteria phage lambda; gene dosage; gene expression regulation; lysogenization; physiology; virus genome; Bacteriophage lambda; Gene Dosage; Gene Expression Regulation, Viral; Genome, Viral; Lysogeny; Models, Biological; Virus Replication
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250921
作者单位	Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, United States; Department of Physics, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; Department of Microbiology, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; Center for the Physics of Living Cells, University of Illinois at Urbana–Champaign, Urbana, IL 61801, United States; Center for Theoretical Biological Physics, Rice University, Houston, TX 77030, United States; Applied Physics Graduate Program, Rice University, Houston, TX 77005, United States; Department of Bioengineering, Rice University, Houston, TX 77030, United States
推荐引用方式 GB/T 7714	Yao T.,Coleman S.,Nguyen T.V.P.,et al. Bacteriophage self-counting in the presence of viral replication[J],2021,118(51).
APA	Yao T.,Coleman S.,Nguyen T.V.P.,Golding I.,&Igoshin O.A..(2021).Bacteriophage self-counting in the presence of viral replication.Proceedings of the National Academy of Sciences of the United States of America,118(51).
MLA	Yao T.,et al."Bacteriophage self-counting in the presence of viral replication".Proceedings of the National Academy of Sciences of the United States of America 118.51(2021).