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DOI	10.1073/pnas.2114952118
	Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana
	Yokochi Y.; Fukushi Y.; Wakabayashi K.-I.; Yoshida K.; Hisabori T.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:51
英文摘要	Thioredoxin (Trx) is a protein that mediates the reducing power transfer from the photosynthetic electron transport system to target enzymes in chloroplasts and regulates their activities. Redox regulation governed by Trx is a system that is central to the adaptation of various chloroplast functions to the ever-changing light environment. However, the factors involved in the opposite reaction (i.e., the oxidation of various enzymes) have yet to be revealed. Recently, it has been suggested that Trx and Trx-like proteins could oxidize Trx-targeted proteins in vitro. To elucidate the in vivo function of these proteins as oxidation factors, we generated mutant plant lines deficient in Trx or Trx-like proteins and studied how the proteins are involved in oxidative regulation in chloroplasts. We found that f-type Trx and two types of Trx-like proteins, Trx-like 2 and atypical Cys His-rich Trx (ACHT), seemed to serve as oxidation factors for Trx-targeted proteins, such as fructose-1,6-bisphosphatase, Rubisco activase, and the γ-subunit of ATP synthase. In addition, ACHT was found to be involved in regulating nonphotochemical quenching, which is the mechanism underlying the thermal dissipation of excess light energy. Overall, these results indicate that Trx and Trx-like proteins regulate chloroplast functions in concert by controlling the redox state of various photosynthesis-related proteins in vivo. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	2-Cys peroxiredoxin; Oxidation; Redox regulation; Thioredoxin; Thioredoxin-like protein
语种	英语
scopus关键词	Arabidopsis protein; thioredoxin; Arabidopsis; chemistry; chloroplast; CRISPR Cas system; enzymology; gene expression regulation; genetics; light; metabolism; mutation; oxidation reduction reaction; physiology; plant leaf; Arabidopsis; Arabidopsis Proteins; Chloroplasts; CRISPR-Cas Systems; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Light; Mutation; Oxidation-Reduction; Plant Leaves; Thioredoxins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250915
作者单位	Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, 226-8503, Japan; School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, 226-8503, Japan
推荐引用方式 GB/T 7714	Yokochi Y.,Fukushi Y.,Wakabayashi K.-I.,et al. Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana[J],2021,118(51).
APA	Yokochi Y.,Fukushi Y.,Wakabayashi K.-I.,Yoshida K.,&Hisabori T..(2021).Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America,118(51).
MLA	Yokochi Y.,et al."Oxidative regulation of chloroplast enzymes by thioredoxin and thioredoxin-like proteins in Arabidopsis thaliana".Proceedings of the National Academy of Sciences of the United States of America 118.51(2021).