气候变化领域集成服务门户(CCPortal): EIN3 and RSL4 interfere with an MYB–bHLH–WD40 complex to mediate ethylene-induced ectopic root hair formation in Arabidopsis

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DOI	10.1073/pnas.2110004118
	EIN3 and RSL4 interfere with an MYB–bHLH–WD40 complex to mediate ethylene-induced ectopic root hair formation in Arabidopsis
	Qiu Y.; Tao R.; Feng Y.; Xiao Z.; Zhang D.; Peng Y.; Wen X.; Wang Y.; Guo H.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:51
英文摘要	The alternating cell specifications of root epidermis to form hair cells or nonhair cells in Arabidopsis are determined by the expression level of GL2, which is activated by an MYB–bHLH–WD40 (WER–GL3–TTG1) transcriptional complex. The phytohormone ethylene (ET) has a unique effect of inducing N-position epidermal cells to form root hairs. However, the molecular mechanisms underlying ET-induced ectopic root hair development remain enigmatic. Here, we show that ET promotes ectopic root hair formation through down-regulation of GL2 expression. ET-activated transcription factors EIN3 and its homolog EIL1 mediate this regulation. Molecular and biochemical analyses further revealed that EIN3 physically interacts with TTG1 and interferes with the interaction between TTG1 and GL3, resulting in reduced activation of GL2 by the WER–GL3–TTG1 complex. Furthermore, we found through genetic analysis that the master regulator of root hair elongation, RSL4, which is directly activated by EIN3, also participates in ET-induced ectopic root hair development. RSL4 negatively regulates the expression of GL2, likely through a mechanism similar to that of EIN3. Therefore, our work reveals that EIN3 may inhibit gene expression by affecting the formation of transcription-activating protein complexes and suggests an unexpected mutual inhibition between the hair elongation factor, RSL4, and the hair specification factor, GL2. Overall, this study provides a molecular framework for the integration of ET signaling and intrinsic root hair development pathway in modulating root epidermal cell specification. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	EIN3; Ethylene; GL2; Root hair; WER–GL3–TTG1 complex
语种	英语
scopus关键词	Arabidopsis protein; basic helix loop helix transcription factor; DNA binding protein; EIL1 protein, Arabidopsis; EIN3 protein, Arabidopsis; ethylene; ethylene derivative; GL2 protein, Arabidopsis; GL3 protein, Arabidopsis; homeodomain protein; multiprotein complex; protein binding; RSL4 protein, Arabidopsis; transcription factor; TTG1 protein, Arabidopsis; WER protein, Arabidopsis; Arabidopsis; cell differentiation; cytology; gene expression regulation; genetics; growth, development and aging; metabolism; plant epidermis; plant root; signal transduction; Arabidopsis; Arabidopsis Proteins; Basic Helix-Loop-Helix Transcription Factors; Cell Differentiation; DNA-Binding Proteins; Ethylenes; Gene Expression Regulation, Plant; Homeodomain Proteins; Multiprotein Complexes; Plant Epidermis; Plant Roots; Protein Binding; Signal Transduction; Transcription Factors
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250911
作者单位	Harbin Institute of Technology, Harbin, 150001, China; Institute of Plant and Food Science, Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, 518055, China; Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, School of Life Sciences, Southern University of Science and Technology, Shenzhen, 518055, China
推荐引用方式 GB/T 7714	Qiu Y.,Tao R.,Feng Y.,等. EIN3 and RSL4 interfere with an MYB–bHLH–WD40 complex to mediate ethylene-induced ectopic root hair formation in Arabidopsis[J],2021,118(51).
APA	Qiu Y..,Tao R..,Feng Y..,Xiao Z..,Zhang D..,...&Guo H..(2021).EIN3 and RSL4 interfere with an MYB–bHLH–WD40 complex to mediate ethylene-induced ectopic root hair formation in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,118(51).
MLA	Qiu Y.,et al."EIN3 and RSL4 interfere with an MYB–bHLH–WD40 complex to mediate ethylene-induced ectopic root hair formation in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 118.51(2021).