气候变化领域集成服务门户(CCPortal): SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes

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DOI	10.1073/pnas.2116668118
	SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes
	Pawlica P.; Yario T.A.; White S.; Wang J.; Moss W.N.; Hui P.; Vinetz J.M.; Steitz J.A.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:52
英文摘要	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease (COVID-19), continues to be a pressing health concern. In this study, we investigated the impact of SARS-CoV-2 infection on host microRNA (miRNA) populations in three human lung-derived cell lines, as well as in nasopharyngeal swabs from SARS-CoV-2-infected individuals. We did not detect any major and consistent differences in host miRNA levels after SARS-CoV-2 infection. However, we unexpectedly discovered a viral miRNA-like small RNA, named CoV2-miR-O7a (for SARS-CoV-2 miRNA-like ORF7a-derived small RNA). Its abundance ranges from low to moderate as compared to host miRNAs and it associates with Argonaute proteins-core components of the RNA interference pathway. We identify putative targets for CoV2-miRO7a, including Basic Leucine Zipper ATF-Like Transcription Factor 2 (BATF2), which participates in interferon signaling. We demonstrate that CoV2-miR-O7a production relies on cellular machinery, yet is independent of Drosha protein, and is enhanced by the presence of a strong and evolutionarily conserved hairpin formed within the ORF7a sequence. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	MicoRNA; Noncoding RNA; SARS-CoV-2
语种	英语
scopus关键词	argonaute protein; basic helix loop helix leucine zipper transcription factor; basic helix loop helix leucine zipper transcription factor 2; Drosha protein; microRNA; ribonucleoprotein; unclassified drug; ORF7a protein, SARS-CoV-2; small untranslated RNA; viral protein; virus RNA; A-549 cell line; Article; Calu-3 cell line; controlled study; coronavirus disease 2019; DNA hairpin; host cell; human; human cell; interferon signaling; nasopharyngeal swab; PC-9 cell line; RNA interference; RNA sequencing; transcription regulation; virus load; gene expression regulation; genetics; host pathogen interaction; metabolism; virology; COVID-19; Gene Expression Regulation, Viral; Host-Pathogen Interactions; Humans; RNA, Small Untranslated; RNA, Viral; SARS-CoV-2; Viral Proteins
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250899
作者单位	Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06536, United States; HHMI, Yale University School of Medicine, New Haven, CT 06536, United States; Department of Pathology, Yale University School of Medicine, New Haven, CT 06536, United States; Roy J. Carver Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 5001, United States; Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06520, United States
推荐引用方式 GB/T 7714	Pawlica P.,Yario T.A.,White S.,et al. SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes[J],2021,118(52).
APA	Pawlica P..,Yario T.A..,White S..,Wang J..,Moss W.N..,...&Steitz J.A..(2021).SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes.Proceedings of the National Academy of Sciences of the United States of America,118(52).
MLA	Pawlica P.,et al."SARS-CoV-2 expresses a microRNA-like small RNA able to selectively repress host genes".Proceedings of the National Academy of Sciences of the United States of America 118.52(2021).