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DOI	10.1073/pnas.2115570119
	The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis
	Potok M.E.; Zhong Z.; Picard C.L.; Liu Q.; Do T.; Jacobsen C.E.; Sakr O.; Naranbaatar B.; Thilakaratne R.; Khnkoyan Z.; Purl M.; Cheng H.; Vervaet H.; Feng S.; Rayatpisheh S.; Wohlschlegel J.A.; O'Malley R.C.; Ecker J.R.; Jacobsen S.E.
发表日期	2022
ISSN	0027-8424
卷号	119 期号:3
英文摘要	ARABIDOPSIS TRITHORAX-RELATED PROTEIN 5 (ATXR5) AND ATXR6 are required for the deposition of H3K27me1 and for maintaining genomic stability in Arabidopsis. Reduction of ATXR5/6 activity results in activation of DNA damage response genes, along with tissue-specific derepression of transposable elements (TEs), chromocenter decompaction, and genomic instability characterized by accumulation of excess DNA from heterochromatin. How loss of ATXR5/6 and H3K27me1 leads to these phenotypes remains unclear. Here we provide extensive characterization of the atxr5/6 hypomorphic mutant by comprehensively examining gene expression and epigenetic changes in the mutant. We found that the tissue-specific phenotypes of TE derepression and excessive DNA in this atxr5/6 mutant correlated with residual ATXR6 expression from the hypomorphic ATXR6 allele. However, up-regulation of DNA damage genes occurred regardless of ATXR6 levels and thus appears to be a separable process. We also isolated an atxr6-null allele which showed that ATXR5 and ATXR6 are required for female germline development. Finally, we characterize three previously reported suppressors of the hypomorphic atxr5/6 mutant and show that these rescue atxr5/6 via distinct mechanisms, two of which involve increasing H3K27me1 levels. © This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).
英文关键词	ATXR5/6; H3K27me1; Histone methyltransferase; Plant
语种	英语
scopus关键词	Arabidopsis trithorax related protein 5; Arabidopsis trithorax related protein 6; DNA; histone lysine methyltransferase; unclassified drug; allele; Arabidopsis; Article; chromatin; controlled study; DNA damage; epigenetics; female germline development; gene expression regulation; gene repression; genetic transcription; genomic instability; nonhuman; phenotype; plant development; plant genome; protein function; tissue specificity; transposon; upregulation
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250890
作者单位	Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095, United States; School of Advanced Agricultural Sciences, Peking University, Beijing, 100871, China; Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, United States; HHMI, The Salk Institute for Biological Studies, La JollaCA 92037, United States; Plant Biology Laboratory, The Salk Institute for Biological Studies, La JollaCA 92037, United States; HHMI, University of California, Los Angeles, CA 90095, United States; Oncology Research and Development, GlaxoSmithKline, Collegeville, PA 19426, United States; Genomic Institute, The Novartis Research Foundation, San Diego, CA 92121, United States; US Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Potok M.E.,Zhong Z.,Picard C.L.,et al. The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis[J],2022,119(3).
APA	Potok M.E..,Zhong Z..,Picard C.L..,Liu Q..,Do T..,...&Jacobsen S.E..(2022).The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America,119(3).
MLA	Potok M.E.,et al."The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis".Proceedings of the National Academy of Sciences of the United States of America 119.3(2022).