气候变化领域集成服务门户(CCPortal): Measuring infectious SARS-CoV-2 in clinical samples reveals a higher viral titer:RNA ratio for Delta and Epsilon vs. Alpha variants

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DOI	10.1073/pnas.2116518119
	Measuring infectious SARS-CoV-2 in clinical samples reveals a higher viral titer:RNA ratio for Delta and Epsilon vs. Alpha variants
	Despres H.W.; Mills M.G.; Shirley D.J.; Schmidt M.M.; Huang M.-L.; Roychoudhury P.; Jerome K.R.; Greninger A.L.; Bruce E.A.
发表日期	2022
ISSN	0027-8424
卷号	119 期号:5
英文摘要	Novel severe acute respiratory syndrome coronavirus 2 (SARSCoV- 2) variants pose a challenge to controlling the COVID-19 pandemic. Previous studies indicate that clinical samples collected from individuals infected with the Delta variant may contain higher levels of RNA than previous variants, but the relationship between levels of viral RNA and infectious virus for individual variants is unknown. We measured infectious viral titer (using a microfocus-forming assay) and total and subgenomic viral RNA levels (using RT-PCR) in a set of 162 clinical samples containing SARS-CoV-2 Alpha, Delta, and Epsilon variants that were collected in identical swab kits from outpatient test sites and processed soon after collection. We observed a high degree of variation in the relationship between viral titers and RNA levels. Despite this, the overall infectivity differed among the three variants. Both Delta and Epsilon had significantly higher infectivity than Alpha, as measured by the number of infectious units per quantity of viral E gene RNA (5.9- and 3.0-fold increase; P < 0.0001, P = 0.014, respectively) or subgenomic E RNA (14.3- and 6.9-fold increase; P < 0.0001, P = 0.004, respectively). In addition to higher viral RNA levels reported for the Delta variant, the infectivity (amount of replication competent virus per viral genome copy) may be increased compared to Alpha. Measuring the relationship between live virus and viral RNA is an important step in assessing the infectivity of novel SARS-CoV-2 variants. An increase in the infectivity for Delta may further explain increased spread, suggesting a need for increased measures to prevent viral transmission. © 2022 National Academy of Sciences. All rights reserved.
英文关键词	Delta; Infectivity; SARS-CoV-2; Variant; Viral load
语种	英语
scopus关键词	genomic RNA; subgenomic RNA; transmembrane protease serine 2; unclassified drug; virus RNA; envelope protein, SARS-CoV-2; virus RNA; Article; Caco-2 cell line; comparative study; controlled study; focus forming assay; Huh-7.5 cell line; human; human cell; nonhuman; outpatient department; plaque forming unit; real time polymerase chain reaction; RNA gene; SARS-CoV-2 Delta; SARS-CoV-2 variant 501Y.V1; SARS-CoV-2 variant CAL.20C; storage temperature; VeroE6/TMPRSS2 cell line; viral E gene; viral RNA blood level; virus infectivity; virus load; virus replication; virus transmission; animal; Chlorocebus aethiops; classification; epidemiology; gene expression regulation; genetics; liver cell; metabolism; pathogenicity; pathology; tumor cell line; Vero cell line; virology; virulence; virus genome; virus load; Animals; Cell Line, Tumor; Chlorocebus aethiops; Coronavirus Envelope Proteins; COVID-19; Gene Expression Regulation, Viral; Genome, Viral; Hepatocytes; Humans; RNA, Viral; SARS-CoV-2; Vero Cells; Viral Load; Virulence
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250880
作者单位	Department of Microbiology and Molecular Genetics, Robert Larner, M.D. College of Medicine, University of Vermont, Burlington, VT 05405, United States; Virology Division, Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98105, United States; Data Science Department, Faraday Inc., Burlington, VT 05405, United States; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, United States
推荐引用方式 GB/T 7714	Despres H.W.,Mills M.G.,Shirley D.J.,et al. Measuring infectious SARS-CoV-2 in clinical samples reveals a higher viral titer:RNA ratio for Delta and Epsilon vs. Alpha variants[J],2022,119(5).
APA	Despres H.W..,Mills M.G..,Shirley D.J..,Schmidt M.M..,Huang M.-L..,...&Bruce E.A..(2022).Measuring infectious SARS-CoV-2 in clinical samples reveals a higher viral titer:RNA ratio for Delta and Epsilon vs. Alpha variants.Proceedings of the National Academy of Sciences of the United States of America,119(5).
MLA	Despres H.W.,et al."Measuring infectious SARS-CoV-2 in clinical samples reveals a higher viral titer:RNA ratio for Delta and Epsilon vs. Alpha variants".Proceedings of the National Academy of Sciences of the United States of America 119.5(2022).