气候变化领域集成服务门户(CCPortal): A standardized genome architecture for bacterial synthetic biology (SEGA)

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DOI	10.1038/s41467-021-26155-5
	A standardized genome architecture for bacterial synthetic biology (SEGA)
	Bayer C.N.; Rennig M.; Ehrmann A.K.; Nørholm M.H.H.
发表日期	2021
ISSN	2041-1723
卷号	12 期号:1
英文摘要	Chromosomal recombinant gene expression offers a number of advantages over plasmid-based synthetic biology. However, the methods applied for bacterial genome engineering are still challenging and far from being standardized. Here, in an attempt to realize the simplest recombinant genome technology imaginable and facilitate the transition from recombinant plasmids to genomes, we create a simplistic methodology and a comprehensive strain collection called the Standardized Genome Architecture (SEGA). In its simplest form, SEGA enables genome engineering by combining only two reagents: a DNA fragment that can be ordered from a commercial vendor and a stock solution of bacterial cells followed by incubation on agar plates. Recombinant genomes are identified by visual inspection using green-white colony screening akin to classical blue-white screening for recombinant plasmids. The modular nature of SEGA allows precise multi-level control of transcriptional, translational, and post-translational regulation. The SEGA architecture simultaneously supports increased standardization of genetic designs and a broad application range by utilizing well-characterized parts optimized for robust performance in the context of the bacterial genome. Ultimately, its adaption and expansion by the scientific community should improve predictability and comparability of experimental outcomes across different laboratories. © 2021, The Author(s).
语种	英语
scopus关键词	agar; DNA fragment; recombinant DNA; bacterium; cell; genome; plasmid; Article; bacterial cell; bacterial genome; cell density; Escherichia coli; flow cytometry; fluorescence activated cell sorting; genetic engineering; genetic manipulation; genetic regulation; nonhuman; plasmid; recombinant plasmid; standardized genome architecture; synthetic biology; translation initiation; bacterium; chromosome; gene expression regulation; gene vector; genetic engineering; genetic recombination; genetics; procedures; promoter region; standard; synthetic biology; Bacteria (microorganisms); Bacteria; Chromosomes; Escherichia coli; Flow Cytometry; Gene Expression Regulation, Bacterial; Genetic Engineering; Genetic Vectors; Genome, Bacterial; Plasmids; Promoter Regions, Genetic; Recombination, Genetic; Reference Standards; Synthetic Biology
来源期刊	Nature Communications
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/250605
作者单位	Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kgs. Lyngby, Denmark
推荐引用方式 GB/T 7714	Bayer C.N.,Rennig M.,Ehrmann A.K.,et al. A standardized genome architecture for bacterial synthetic biology (SEGA)[J],2021,12(1).
APA	Bayer C.N.,Rennig M.,Ehrmann A.K.,&Nørholm M.H.H..(2021).A standardized genome architecture for bacterial synthetic biology (SEGA).Nature Communications,12(1).
MLA	Bayer C.N.,et al."A standardized genome architecture for bacterial synthetic biology (SEGA)".Nature Communications 12.1(2021).