气候变化领域集成服务门户(CCPortal): High-throughput, semi-automated dithiothreitol (DTT) assays for oxidative potential of fine particulate matter

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DOI	10.1016/j.atmosenv.2019.117132
	High-throughput, semi-automated dithiothreitol (DTT) assays for oxidative potential of fine particulate matter
	Berg K.E.; Clark K.M.; Li X.; Carter E.M.; Volckens J.; Henry C.S.
发表日期	2020
ISSN	1352-2310
卷号	222
英文摘要	Fine particulate matter (PM2.5) air pollution exposure is a leading risk factor for adverse health outcomes, including cardiovascular and respiratory morbidity, and premature mortality. Quantification of PM2.5 oxidative potential (i.e., the ability of PM to promote oxidative reactions in solution) is a relatively new paradigm for exploring health risks associated with the various chemical compositions of ambient PM2.5. PM2.5 oxidative potential is commonly measured with the dithiothreitol (DTT) assay, where the DTT loss rate is measured when mixed with a PM2.5 sample extract. However, the DTT assay is time consuming and laborious, with only a few reported automation attempts. We introduce and evaluate a semi-automated DTT assay using a traditional HPLC combined with either UV/vis absorbance or electrochemical detection that has comparable accuracy and sensitivity to manual approaches. Commercial and custom-made electrochemical detectors are also compared before measuring ambient PM2.5 filter samples. The optimized, semi-automated assay can process six samples per hour (an 83% time savings compared to manual analysis). Cost becomes significant for large-scale studies and was also considered; electrochemical detection saved 40% on consumables cost compared to UV/vis detection. The presented liquid-handling automation can be applied to a variety of autosamplers in other laboratories for DTT assay semi-automation. © 2019
关键词	Automated Dithiothreitol Electrochemical Oxidative potential UV/vis absorbance
语种	英语
scopus关键词	Chemical detection; Health risks; Particles (particulate matter); Absorbances; Automated; Dithiothreitol; Electrochemical; Oxidative potential; Automation; dithiothreitol; absorbance; atmospheric pollution; bioassay; cardiovascular disease; chemical compound; electrochemical method; health risk; morbidity; mortality; oxidation; particulate matter; public health; respiratory disease; risk factor; ultraviolet radiation; aerobic metabolism; air pollution; automation; cardiovascular mortality; chemical composition; controlled study; electrochemical detection; health hazard; high performance liquid chromatography; limit of quantitation; morbidity; oxidation reduction potential; particulate matter; premature mortality; priority journal; rate constant; room temperature
来源期刊	ATMOSPHERIC ENVIRONMENT
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/249454
作者单位	Department of Chemistry, Colorado State University, Fort Collins, CO 80523, United States; Department of Civil & Environmental Engineering, Colorado State University, Fort Collins, CO 80523, United States; Department of Mechanical Engineering, Colorado State University, Fort Collins, CO 80523, United States; Department of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, United States; Department of Chemical & Biological Engineering, Colorado State University, Fort Collins, CO 80523, United States
推荐引用方式 GB/T 7714	Berg K.E.,Clark K.M.,Li X.,et al. High-throughput, semi-automated dithiothreitol (DTT) assays for oxidative potential of fine particulate matter[J],2020,222.
APA	Berg K.E.,Clark K.M.,Li X.,Carter E.M.,Volckens J.,&Henry C.S..(2020).High-throughput, semi-automated dithiothreitol (DTT) assays for oxidative potential of fine particulate matter.ATMOSPHERIC ENVIRONMENT,222.
MLA	Berg K.E.,et al."High-throughput, semi-automated dithiothreitol (DTT) assays for oxidative potential of fine particulate matter".ATMOSPHERIC ENVIRONMENT 222(2020).