气候变化领域集成服务门户(CCPortal): Dynamic control of plant water use using designed ABA receptor agonists

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DOI	10.1126/science.aaw8848
	Dynamic control of plant water use using designed ABA receptor agonists
	Vaidya A.S.; Helander J.D.M.; Peterson F.C.; Elzinga D.; Dejonghe W.; Kaundal A.; Park S.-Y.; Xing Z.; Mega R.; Takeuchi J.; Khanderahoo B.; Bishay S.; Volkman B.F.; Todoroki Y.; Okamoto M.; Cutler S.R.
发表日期	2019
ISSN	0036-8075
卷号	366 期号:6464
英文摘要	Drought causes crop losses worldwide, and its impact is expected to increase as the world warms. This has motivated the development of small-molecule tools for mitigating the effects of drought on agriculture. We show here that current leads are limited by poor bioactivity in wheat, a widely grown staple crop, and in tomato. To address this limitation, we combined virtual screening, x-ray crystallography, and structure-guided design to develop opabactin (OP), an abscisic acid (ABA) mimic with up to an approximately sevenfold increase in receptor affinity relative to ABA and up to 10-fold greater activity in vivo. Studies in Arabidopsis thaliana reveal a role of the type III receptor PYRABACTIN RESISTANCE-LIKE 2 for the antitranspirant efficacy of OP. Thus, virtual screening and structure-guided optimization yielded newly discovered agonists for manipulating crop abiotic stress tolerance and water use. © 2019 American Association for the Advancement of Science. All rights reserved.
英文关键词	abscisic acid; hormone receptor stimulating agent; molecular scaffold; opabactin; unclassified drug; abscisic acid; Arabidopsis protein; cell surface receptor; hormone; PYL10 protein, Arabidopsis; water; abscisic acid; agrometeorology; bioactivity; drought stress; ecophysiology; experimental study; optimization; protein; water use efficiency; Arabidopsis; Article; barley; binding affinity; chemical structure; Commelina; controlled study; crystal structure; germination; in vitro study; molecular docking; nonhuman; plant water use; priority journal; protein binding; protein function; protein modification; reaction optimization; signal transduction; thermodynamics; tomato; wheat; chemistry; drought; drug effect; evapotranspiration; molecular model; physiological stress; physiology; Arabidopsis thaliana; Lycopersicon esculentum; Triticum aestivum; Abscisic Acid; Arabidopsis; Arabidopsis Proteins; Droughts; Hormones; Lycopersicon esculentum; Models, Molecular; Plant Transpiration; Receptors, Cell Surface; Stress, Physiological; Triticum; Water
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245813
作者单位	Institute for Integrative Genome Biology, University of California, Riverside, Riverside, CA 92521, United States; Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA 92521, United States; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI 53226, United States; Department of Plants, Soils and Climate, Utah State University, Logan, UT 84322, United States; Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori, 680-0001, Japan; Faculty of Agriculture, Shizuoka University, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan; Center for Bioscience Research and Education, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi, 321-8505, Japan; PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan
推荐引用方式 GB/T 7714	Vaidya A.S.,Helander J.D.M.,Peterson F.C.,et al. Dynamic control of plant water use using designed ABA receptor agonists[J],2019,366(6464).
APA	Vaidya A.S..,Helander J.D.M..,Peterson F.C..,Elzinga D..,Dejonghe W..,...&Cutler S.R..(2019).Dynamic control of plant water use using designed ABA receptor agonists.Science,366(6464).
MLA	Vaidya A.S.,et al."Dynamic control of plant water use using designed ABA receptor agonists".Science 366.6464(2019).