气候变化领域集成服务门户(CCPortal): RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction

CCPortal

DOI	10.1126/science.abe6523
	RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction
	Torabi S.-F.; Vaidya A.T.; Tycowski K.T.; DeGregorio S.J.; Wang J.; Shu M.-D.; Steitz T.A.; Steitz J.A.
发表日期	2021
ISSN	0036-8075
卷号	371 期号:6529
英文摘要	Polyadenylate [poly(A)] tail addition to the 3' end of a wide range of RNAs is a highly conserved modification that plays a central role in cellular RNA function. Elements for nuclear expression (ENEs) are cis-acting RNA elements that stabilize poly(A) tails by sequestering them in RNA triplex structures. A crystal structure of a double ENE from a rice hAT transposon messenger RNA complexed with poly(A)28at a resolution of 2.89 angstroms reveals multiple modes of interaction with poly(A), including major-groove triple helices, extended minor-groove interactions with RNA double helices, a quintuple-base motif that transitions poly(A) from minor-groove associations to major-groove triple helices, and a poly(A) 3'-end binding pocket. Our findings both expand the repertoire of motifs involved in long-range RNA interactions and provide insights into how polyadenylation can protect an RNA's extreme 3' end. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	double stranded RNA; messenger RNA; polyadenylic acid; polyadenylic acid; chemical binding; crystal structure; polymer; RNA; stabilization; 3' untranslated region; Article; cell nucleus; crystal structure; crystallization; gene expression; gene function; genetic conservation; molecular interaction; polyadenylation; protein motif; RNA binding; RNA stability; RNA structure; structural homology; structure analysis; transposon; chemistry; conformation; Oryza; polyadenylation; Crystallization; Nucleic Acid Conformation; Oryza; Poly A; Polyadenylation; RNA Stability; RNA, Messenger
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245768
作者单位	Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, CT 06536, United States; Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06536, United States; TIFR Centre for Interdisciplinary Sciences, Tata Institute of Fundamental Research, Hyderabad, 500046, India
推荐引用方式 GB/T 7714	Torabi S.-F.,Vaidya A.T.,Tycowski K.T.,et al. RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction[J],2021,371(6529).
APA	Torabi S.-F..,Vaidya A.T..,Tycowski K.T..,DeGregorio S.J..,Wang J..,...&Steitz J.A..(2021).RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction.Science,371(6529).
MLA	Torabi S.-F.,et al."RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction".Science 371.6529(2021).