气候变化领域集成服务门户(CCPortal): Single-cell nuclear architecture across cell types in the mouse brain

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DOI	10.1126/science.abj1966
	Single-cell nuclear architecture across cell types in the mouse brain
	Takei Y.; Zheng S.; Yun J.; Shah S.; Pierson N.; White J.; Schindler S.; Tischbirek C.H.; Yuan G.-C.; Cai L.
发表日期	2021
ISSN	0036-8075
起始页码	586
结束页码	594
卷号	374 期号:6567
英文摘要	Diverse cell types in tissues have distinct gene expression programs, chromatin states, and nuclear architectures. To correlate such multimodal information across thousands of single cells in mouse brain tissue sections, we use integrated spatial genomics, imaging thousands of genomic loci along with RNAs and epigenetic markers simultaneously in individual cells. We reveal that cell type-specific association and scaffolding of DNA loci around nuclear bodies organize the nuclear architecture and correlate with differential expression levels in different cell types. At the submegabase level, active and inactive X chromosomes access similar domain structures in single cells despite distinct epigenetic and expression states. This work represents a major step forward in linking single-cell three-dimensional nuclear architecture, gene expression, and epigenetic modifications in a native tissue context. Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works
英文关键词	brain; cell component; detection method; DNA; gene expression; animal cell; animal experiment; animal tissue; article; brain tissue; cell culture; cell nucleus inclusion body; chromatin structure; epigenetic modification; epigenetics; gene expression; genetic marker; genomics; male; mouse; nonhuman; tissue section; X chromosome; brain
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245702
作者单位	Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, United States; Department of Genetics and Genomic Sciences, Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States
推荐引用方式 GB/T 7714	Takei Y.,Zheng S.,Yun J.,et al. Single-cell nuclear architecture across cell types in the mouse brain[J],2021,374(6567).
APA	Takei Y..,Zheng S..,Yun J..,Shah S..,Pierson N..,...&Cai L..(2021).Single-cell nuclear architecture across cell types in the mouse brain.Science,374(6567).
MLA	Takei Y.,et al."Single-cell nuclear architecture across cell types in the mouse brain".Science 374.6567(2021).