气候变化领域集成服务门户(CCPortal): Activity-dependent spatially localized miRNA maturation in neuronal dendrites

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DOI	10.1126/science.aaf8995
	Activity-dependent spatially localized miRNA maturation in neuronal dendrites
	Sambandan S.; Akbalik G.; Kochen L.; Rinne J.; Kahlstatt J.; Glock C.; Tushev G.; Alvarez-Castelao B.; Heckel A.; Schuman E.M.
发表日期	2017
ISSN	0036-8075
起始页码	634
结束页码	637
卷号	355 期号:6325
英文摘要	MicroRNAs (miRNAs) regulate gene expression by binding to target messenger RNAs (mRNAs) and preventing their translation. In general, the number of potential mRNA targets in a cell is much greater than the miRNA copy number, complicating high-fidelity miRNA-target interactions. We developed an inducible fluorescent probe to explore whether the maturation of a miRNA could be regulated in space and time in neurons. A precursor miRNA (pre-miRNA) probe exhibited an activity-dependent increase in fluorescence, suggesting the stimulation of miRNA maturation. Single-synapse stimulation resulted in a local maturation of miRNA that was associated with a spatially restricted reduction in the protein synthesis of a target mRNA. Thus, the spatially and temporally regulated maturation of pre-miRNAs can be used to increase the precision and robustness of miRNA-mediated translational repression. © 2017, American Association for the Advancement of Science. All rights reserved.
英文关键词	fluorescent dye; microRNA; messenger RNA; microRNA; MIRN181 microRNA, rat; ribonuclease III; cells and cell components; gene expression; genetic analysis; maturation; mutation; protein; RNA; animal cell; Article; controlled study; dendrite; dendritic spine; fluorescence; microRNA maturation; nerve cell; nonhuman; nucleic acid structure, metabolism and function; priority journal; protein synthesis; rat; RNA processing; RNA translation; animal; cell culture; chemistry; cytology; dendrite; gene expression regulation; genetics; hippocampus; male; metabolism; nerve cell; RNA cleavage; Sprague Dawley rat; synapse; Animals; Cells, Cultured; Dendrites; Fluorescent Dyes; Gene Expression Regulation; Hippocampus; Male; MicroRNAs; Neurons; Protein Biosynthesis; Rats; Rats, Sprague-Dawley; Ribonuclease III; RNA Cleavage; RNA, Messenger; Synapses
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/245328
作者单位	Max Planck Institute for Brain Research, Max-von-Laue Straße 4, Frankfurt, 60438, Germany; Institute for Organic Chemistry and Chemical Biology, Goethe University, Frankfurt, 60438, Germany; Cluster of Excellence Macromolecular Complexes in Action, Goethe University, Frankfurt, 60438, Germany
推荐引用方式 GB/T 7714	Sambandan S.,Akbalik G.,Kochen L.,et al. Activity-dependent spatially localized miRNA maturation in neuronal dendrites[J],2017,355(6325).
APA	Sambandan S..,Akbalik G..,Kochen L..,Rinne J..,Kahlstatt J..,...&Schuman E.M..(2017).Activity-dependent spatially localized miRNA maturation in neuronal dendrites.Science,355(6325).
MLA	Sambandan S.,et al."Activity-dependent spatially localized miRNA maturation in neuronal dendrites".Science 355.6325(2017).