气候变化领域集成服务门户(CCPortal): Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity

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DOI	10.1126/science.abe4713
	Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity
	Li J.; Halitschke R.; Li D.; Paetz C.; Su H.; Heiling S.; Xu S.; Baldwin I.T.
发表日期	2021
ISSN	0036-8075
起始页码	255
结束页码	260
卷号	371 期号:6526
英文摘要	Many plant specialized metabolites function in herbivore defense, and abrogating particular steps in their biosynthetic pathways frequently causes autotoxicity. However, the molecular mechanisms underlying their defense and autotoxicity remain unclear. Here, we show that silencing two cytochrome P450s involved in diterpene biosynthesis in the wild tobacco Nicotiana attenuata causes severe autotoxicity symptoms that result from the inhibition of sphingolipid biosynthesis by noncontrolled hydroxylated diterpene derivatives. Moreover, the diterpenes' defensive function is achieved by inhibiting herbivore sphingolipid biosynthesis through postingestive backbone hydroxylation products. Thus, by regulating metabolic modifications, tobacco plants avoid autotoxicity and gain herbivore defense. The postdigestive duet that occurs between plants and their insect herbivores can reflect the plant's solutions to the "toxic waste dump"problem of using potent chemical defenses. © 2021 The Authors, some rights reserved.
英文关键词	ceramide; ceramide synthase; cytochrome P450; diterpenoid; geranyllinalool; linalool; sphingolipid; unclassified drug; dihydroceramide desaturase; diterpenoid; glucoside; oxidoreductase; chemical compound; chemical defense; chemical reaction; ecotoxicology; Article; autotoxicity; biosynthesis; chemical structure; gene silencing; hydroxylation; metabolomics; microarray analysis; Nicotiana; Nicotiana attenuata; nonhuman; plant defense; plant defense against herbivores; plant leaf; priority journal; structure analysis; symptom; toxicity; whole genome sequencing; animal; biosynthesis; enzymology; genetics; herbivory; hydroxylation; Manduca; metabolism; physiology; tobacco; Hexapoda; Nicotiana attenuata; Nicotiana rustica; Nicotiana tabacum; Animals; Cytochrome P-450 Enzyme System; Diterpenes; Glucosides; Herbivory; Hydroxylation; Manduca; Oxidoreductases; Sphingolipids; Tobacco
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244530
作者单位	Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, Jena, D-07745, Germany; Department of Biosynthesis/NMR, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, Jena, D-07745, Germany; Institute for Evolution and Biodiversity, University of Münster, Hüfferstrasse 1, Münster, D-48161, Germany
推荐引用方式 GB/T 7714	Li J.,Halitschke R.,Li D.,et al. Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity[J],2021,371(6526).
APA	Li J..,Halitschke R..,Li D..,Paetz C..,Su H..,...&Baldwin I.T..(2021).Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity.Science,371(6526).
MLA	Li J.,et al."Controlled hydroxylations of diterpenoids allow for plant chemical defense without autotoxicity".Science 371.6526(2021).