气候变化领域集成服务门户(CCPortal): Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts

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DOI	10.1126/science.abg2166
	Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts
	LeGault K.N.; Hays S.G.; Angermeyer A.; McKitterick A.C.; Johura F.-T.; Sultana M.; Ahmed T.; Alam M.; Seed K.D.
发表日期	2021
ISSN	0036-8075
卷号	373 期号:6554
英文摘要	Bacteriophage predation selects for diverse antiphage systems that frequently cluster on mobilizable defense islands in bacterial genomes. However, molecular insight into the reciprocal dynamics of phage-bacterial adaptations in nature is lacking, particularly in clinical contexts where there is need to inform phage therapy efforts and to understand how phages drive pathogen evolution. Using time-shift experiments, we uncovered fluctuations in Vibrio cholerae's resistance to phages in clinical samples. We mapped phage resistance determinants to SXT integrative and conjugative elements (ICEs), which notoriously also confer antibiotic resistance. We found that SXT ICEs, which are widespread in g-proteobacteria, invariably encode phage defense systems localized to a single hotspot of genetic exchange. We identified mechanisms that allow phage to counter SXT-mediated defense in clinical samples, and document the selection of a novel phage-encoded defense inhibitor. Phage infection stimulates highfrequency SXT ICE conjugation, leading to the concurrent dissemination of phage and antibiotic resistances. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	adaptation; antibiotic resistance; bacterioplankton; detection method; evolution; host-pathogen interaction; pathogen; predation; antibiotic resistance; article; bacteriophage; conjugation; controlled study; gene transfer; infectious agent; nonhuman; Vibrio cholerae; antibiotic resistance; bacterial gene; bacterial genome; bacteriolysis; bacterium conjugation; cholera; drug effect; feces; Gammaproteobacteria; genetic epigenesis; genetics; host range; human; interspersed repeat; isolation and purification; metabolism; microbiology; Myoviridae; organismal interaction; physiology; virology; virus gene; virus genome; Bacteria (microorganisms); Proteobacteria; Vibrio cholerae; viral protein; Bacteriolysis; Cholera; Conjugation, Genetic; Drug Resistance, Bacterial; Epigenesis, Genetic; Feces; Gammaproteobacteria; Genes, Bacterial; Genes, Viral; Genome, Bacterial; Genome, Viral; Host Specificity; Humans; Interspersed Repetitive Sequences; Microbial Interactions; Myoviridae; Vibrio cholerae; Viral Proteins
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244489
作者单位	Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States; Icddr,b, International Centre for Diarrhoeal Disease Research Bangladesh, Dhaka, Bangladesh; Chan Zuckerberg Biohub, San Francisco, CA 94158, United States
推荐引用方式 GB/T 7714	LeGault K.N.,Hays S.G.,Angermeyer A.,et al. Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts[J],2021,373(6554).
APA	LeGault K.N..,Hays S.G..,Angermeyer A..,McKitterick A.C..,Johura F.-T..,...&Seed K.D..(2021).Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts.Science,373(6554).
MLA	LeGault K.N.,et al."Temporal shifts in antibiotic resistance elements govern phage-pathogen conflicts".Science 373.6554(2021).