气候变化领域集成服务门户(CCPortal): Replication timing maintains the global epigenetic state in human cells

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DOI	10.1126/science.aba5545
	Replication timing maintains the global epigenetic state in human cells
	Klein K.N.; Zhao P.A.; Lyu X.; Sasaki T.; Bartlett D.A.; Singh A.M.; Tasan I.; Zhang M.; Watts L.P.; Hiraga S.-I.; Natsume T.; Zhou X.; Baslan T.; Leung D.; Kanemaki M.T.; Donaldson A.D.; Zhao H.; Dalton S.; Corces V.G.; Gilbert D.M.
发表日期	2021
ISSN	0036-8075
起始页码	371
结束页码	378
卷号	372 期号:6540
英文摘要	The temporal order of DNA replication [replication timing (RT)] is correlated with chromatin modifications and three-dimensional genome architecture; however, causal links have not been established, largely because of an inability to manipulate the global RT program. We show that loss of RIF1 causes near-complete elimination of the RT program by increasing heterogeneity between individual cells. RT changes are coupled with widespread alterations in chromatin modifications and genome compartmentalization. Conditional depletion of RIF1 causes replication-dependent disruption of histone modifications and alterations in genome architecture. These effects were magnified with successive cycles of altered RT. These results support models in which the timing of chromatin replication and thus assembly plays a key role in maintaining the global epigenetic state. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	cell; compartmentalization; DNA; genome; heterogeneity; article; chromatin assembly and disassembly; DNA replication timing; epigenetics; histone modification; human; human cell; cell line; chromatin; DNA replication; gene expression; gene knockout; genetic epigenesis; genetics; heterochromatin; histone code; human genome; metabolism; histone; Rif1 protein, human; telomere binding protein; Cell Line; Chromatin; Chromatin Assembly and Disassembly; DNA Replication; DNA Replication Timing; Epigenesis, Genetic; Epigenome; Gene Expression; Gene Knockout Techniques; Genome, Human; Heterochromatin; Histone Code; Histones; Humans; Telomere-Binding Proteins
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/244358
作者单位	Department of Biological Science, Florida State University, Tallahassee, FL 32306, United States; Department of Human Genetics, Emory University, School of Medicine, Atlanta, GA 30322, United States; State Key Laboratory of Cellular Stress Biology, Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, China; San Diego Biomedical Research Institute, San Diego, CA 92121, United States; Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, United States; Institute of Medical Sciences, University of Aberdeen, Aberdeen, AB25 2ZD, United Kingdom; Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS), Yata 1111, Mishima, Shizuoka, 411-8540, Japan; Department of Genetics, The Graduate University for Advanced Studies (SO...
推荐引用方式 GB/T 7714	Klein K.N.,Zhao P.A.,Lyu X.,et al. Replication timing maintains the global epigenetic state in human cells[J],2021,372(6540).
APA	Klein K.N..,Zhao P.A..,Lyu X..,Sasaki T..,Bartlett D.A..,...&Gilbert D.M..(2021).Replication timing maintains the global epigenetic state in human cells.Science,372(6540).
MLA	Klein K.N.,et al."Replication timing maintains the global epigenetic state in human cells".Science 372.6540(2021).