气候变化领域集成服务门户(CCPortal): Imaging translation dynamics in live embryos reveals spatial heterogeneities

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DOI	10.1126/science.abc3483
	Imaging translation dynamics in live embryos reveals spatial heterogeneities
	Dufourt J.; Bellec M.; Trullo A.; Dejean M.; De Rossi S.; Favard C.; Lagha M.
发表日期	2021
ISSN	0036-8075
起始页码	840
结束页码	844
卷号	372 期号:6544
英文摘要	Much is known about the factors involved in the translation of messenger RNA (mRNA) into protein; however, this multistep process has not been imaged in living multicellular organisms. Here, we deploy the SunTag method to visualize and quantify the timing, location, and kinetics of the translation of single mRNAs in living Drosophila embryos. By focusing on the translation of the conserved major epithelial-mesenchymal transition-inducing transcription factor Twist, we identify spatial heterogeneity in mRNA translation efficiency and reveal the existence of translation factories, where clustered mRNAs are cotranslated preferentially at basal perinuclear regions. Observing the location and dynamics of mRNA translation in a living multicellular organism opens avenues for understanding gene regulation during development. Copyright © 2021 The Authors, some rights reserved.
英文关键词	messenger RNA; transcription factor Twist; messenger RNA; embryo; heterogeneity; imaging method; spatial analysis; animal experiment; Article; clustered regularly interspaced short palindromic repeat; controlled study; Drosophila; embryo; embryo development; epithelial mesenchymal transition; gene control; gene expression; nonhuman; quantitative analysis; reporter gene; RNA translation; transgene; animal; Drosophila melanogaster; embryology; genetics; kinetics; metabolism; nonmammalian embryo; procedures; protein synthesis; single molecule imaging; Animals; Drosophila melanogaster; Embryo, Nonmammalian; Kinetics; Protein Biosynthesis; RNA, Messenger; Single Molecule Imaging
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243582
作者单位	Institut de Génétique Moléculaire de Montpellier, University of Montpellier, CNRS-UMR 5535, Montpellier, cedex 5, 34293, France; MRI, BioCampus Montpellier, CNRS, INSERM, University of Montpellier, Montpellier, France; Institut de Recherche en Infectiologie de Montpellier, CNRS UMR 9004, University of Montpellier, Montpellier, cedex 5, 34293, France
推荐引用方式 GB/T 7714	Dufourt J.,Bellec M.,Trullo A.,et al. Imaging translation dynamics in live embryos reveals spatial heterogeneities[J],2021,372(6544).
APA	Dufourt J..,Bellec M..,Trullo A..,Dejean M..,De Rossi S..,...&Lagha M..(2021).Imaging translation dynamics in live embryos reveals spatial heterogeneities.Science,372(6544).
MLA	Dufourt J.,et al."Imaging translation dynamics in live embryos reveals spatial heterogeneities".Science 372.6544(2021).