气候变化领域集成服务门户(CCPortal): Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides

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DOI	10.1126/science.aav0748
	Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides
	Buscaill P.; Chandrasekar B.; Sanguankiattichai N.; Kourelis J.; Kaschani F.; Thomas E.L.; Morimoto K.; Kaiser M.; Preston G.M.; Ichinose Y.; Van Der Hoorn R.A.L.
发表日期	2019
ISSN	0036-8075
卷号	364 期号:6436
英文摘要	Plants and animals recognize conserved flagellin fragments as a signature of bacterial invasion. These immunogenic elicitor peptides are embedded in the flagellin polymer and require hydrolytic release before they can activate cell surface receptors. Although much of flagellin signaling is understood, little is known about the release of immunogenic fragments. We discovered that plant-secreted b-galactosidase 1 (BGAL1) of Nicotiana benthamiana promotes hydrolytic elicitor release and acts in immunity against pathogenic Pseudomonas syringae strains only when they carry a terminal modified viosamine (mVio) in the flagellin O-glycan. In counter defense, P. syringae pathovars evade host immunity by using BGAL1-resistant O-glycans or by producing a BGAL1 inhibitor. Polymorphic glycans on flagella are common to plant and animal pathogenic bacteria and represent an important determinant of host immunity to bacterial pathogens. © 2019 American Association for the Advancement of Science. All rights reserved.
英文关键词	beta galactosidase; flagellin; glycan; glycosidase; reactive oxygen metabolite; rhamnose; flagellin; polymer; polymerized flagellin; polysaccharide; enzyme; enzyme activity; hydrolysis; immune response; immunity; inhibitor; peptide; polymer; polymorphism; protein; apoplast; Article; bacterial flagellum; bacterial gene; controlled study; enzyme activity; fliC gene; gene editing; hydrolysis; immunogenicity; mutant; Nicotiana benthamiana; nonhuman; plant immunity; priority journal; protein polymorphism; protein secretion; Pseudomonas syringae pv. syringae; Pseudomonas syringae pv. tabaci; Pseudomonas syringae pv. tomato; chemistry; enzymology; genetics; glycosylation; host pathogen interaction; immunology; metabolism; microbiology; pathogenicity; Pseudomonas syringae; tobacco; Animalia; Bacteria (microorganisms); Nicotiana benthamiana; Pseudomonas syringae; beta-Galactosidase; Flagellin; Glycosylation; Host-Pathogen Interactions; Hydrolysis; Polymers; Polysaccharides; Pseudomonas syringae; Tobacco
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243201
作者单位	Department of Plant Sciences, University of Oxford, Oxford, United Kingdom; ZMB Chemical Biology, Faculty of Biology, University of Duisburg-Essen, Essen, Germany; Graduate School of Environmental and Life Science, Okayama University, Japan
推荐引用方式 GB/T 7714	Buscaill P.,Chandrasekar B.,Sanguankiattichai N.,et al. Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides[J],2019,364(6436).
APA	Buscaill P..,Chandrasekar B..,Sanguankiattichai N..,Kourelis J..,Kaschani F..,...&Van Der Hoorn R.A.L..(2019).Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides.Science,364(6436).
MLA	Buscaill P.,et al."Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides".Science 364.6436(2019).