气候变化领域集成服务门户(CCPortal): Sleep-wake cycles drive daily dynamics of synaptic phosphorylation

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DOI	10.1126/science.aav3617
	Sleep-wake cycles drive daily dynamics of synaptic phosphorylation
	Brüning F.; Noya S.B.; Bange T.; Koutsouli S.; Rudolph J.D.; Tyagarajan S.K.; Cox J.; Mann M.; Brown S.A.; Robles M.S.
发表日期	2019
ISSN	0036-8075
卷号	366 期号:6462
英文摘要	The circadian clock drives daily changes of physiology, including sleep-wake cycles, through regulation of transcription, protein abundance, and function. Circadian phosphorylation controls cellular processes in peripheral organs, but little is known about its role in brain function and synaptic activity. We applied advanced quantitative phosphoproteomics to mouse forebrain synaptoneurosomes isolated across 24 hours, accurately quantifying almost 8000 phosphopeptides. Half of the synaptic phosphoproteins, including numerous kinases, had large-amplitude rhythms peaking at rest-activity and activity-rest transitions. Bioinformatic analyses revealed global temporal control of synaptic function through phosphorylation, including synaptic transmission, cytoskeleton reorganization, and excitatory/inhibitory balance. Sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures. © 2019 American Association for the Advancement of Science. All rights reserved.
英文关键词	brain peptide; ion channel; phosphopeptide; phosphotransferase; proteome; receptor; scaffold protein; phosphoprotein; phosphotransferase; brain; chemical reaction; circadian rhythm; physiology; protein; sleep; adult; animal experiment; animal tissue; Article; circadian rhythm; controlled study; enzyme activity; enzyme phosphorylation; enzyme regulation; forebrain; male; mass spectrometry; nonhuman; priority journal; protein function; protein phosphorylation; protein protein interaction; proteomics; quantitative analysis; signal transduction; sleep deprivation; sleep waking cycle; synaptic inhibition; synaptic potential; synaptic transmission; animal; C57BL mouse; metabolism; mouse; phosphorylation; sleep; synapse; wakefulness; Animals; Circadian Clocks; Circadian Rhythm; Male; Mice; Mice, Inbred C57BL; Phosphoproteins; Phosphorylation; Phosphotransferases; Prosencephalon; Sleep; Synapses; Wakefulness
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243178
作者单位	Institute of Medical Psychology, Faculty of Medicine, LMU Munich, Germany; Department of Proteomics and Signal Transduction, Max-Planck Institute of Biochemistry, Martinsried, 82152, Germany; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland; Computational Systems Biochemistry, Max-Planck Institute of Biochemistry, Martinsried, Germany; Novo Nordisk Foundation Center for Protein Research, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark
推荐引用方式 GB/T 7714	Brüning F.,Noya S.B.,Bange T.,et al. Sleep-wake cycles drive daily dynamics of synaptic phosphorylation[J],2019,366(6462).
APA	Brüning F..,Noya S.B..,Bange T..,Koutsouli S..,Rudolph J.D..,...&Robles M.S..(2019).Sleep-wake cycles drive daily dynamics of synaptic phosphorylation.Science,366(6462).
MLA	Brüning F.,et al."Sleep-wake cycles drive daily dynamics of synaptic phosphorylation".Science 366.6462(2019).