气候变化领域集成服务门户(CCPortal): Advances and challenges in time-resolved macromolecular crystallography

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DOI	10.1126/science.aba0954
	Advances and challenges in time-resolved macromolecular crystallography
	Brändén G.; Neutze R.
发表日期	2021
ISSN	0036-8075
卷号	373 期号:6558
英文摘要	Conformational changes within biological macromolecules control a vast array of chemical reactions in living cells. Time-resolved crystallography can reveal time-dependent structural changes that occur within protein crystals, yielding chemical insights in unparalleled detail. Serial crystallography approaches developed at x-ray free-electron lasers are now routinely used for time-resolved diffraction studies of macromolecules. These techniques are increasingly being applied at synchrotron radiation sources and to a growing diversity of macromolecules. Here, we review recent progress in the field, including visualizing ultrafast structural changes that guide the initial trajectories of light-driven reactions as well as capturing biologically important conformational changes on slower time scales, for which bacteriorhodopsin and photosystem II are presented as illustrative case studies. © 2021 American Association for the Advancement of Science. All rights reserved.
英文关键词	array; crystallography; diffraction; time
语种	英语
来源期刊	Science
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/243135
作者单位	Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden
推荐引用方式 GB/T 7714	Brändén G.,Neutze R.. Advances and challenges in time-resolved macromolecular crystallography[J],2021,373(6558).
APA	Brändén G.,&Neutze R..(2021).Advances and challenges in time-resolved macromolecular crystallography.Science,373(6558).
MLA	Brändén G.,et al."Advances and challenges in time-resolved macromolecular crystallography".Science 373.6558(2021).