气候变化领域集成服务门户(CCPortal): The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299

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DOI	10.1089/cbr.2011.1037
	The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299
	Xu, Hua; Gao, Lan; Che, Tuanjie; Du, Wenjing; Li, Qiang; Gao, Qingxiang; Wang, Ziren
发表日期	2012
ISSN	1084-9785
EISSN	1557-8852
起始页码	113
结束页码	118
卷号	27 期号:2
英文摘要	Introduction: We aimed to investigate the effects of C-12(6+) irradiation on the cell cycle and apoptosis as well as the associated mechanisms in the human lung cancer cell line H1299. Methods: After irradiation with different doses of C-12(6+) for varying lengths of incubation, the changes in H1299 cells were assayed by flow cytometry and the microculture tetrazolium test. The expression of caspase-3 was detected by immunocytochemistry, western blot, and reverse transcription-polymerase chain reaction (RT-PCR). Results: The G(2)/M phase was blocked after treatment with 1 and 2 Gy at the 12-hour time point, and the most obvious block of G(2)/M occurred after treatment with 2 and 4 Gy at the 24-hour time point in a dose-dependent manner. The apoptosis rate increased with increasing radiation dose and reached a peak after the cells were irradiated with 2 Gy and incubated for 48 hours. In addition, the RT-PCR, western blot, and ICC results showed that irradiation with C-12(6+) significantly increased the expression of caspase-3 compared with the control group (p < 0.05). Conclusions: Irradiation of H1299 cells with C-12(6+) induced apoptosis and significantly inhibited their growth through heavy ion irradiation-mediated activation of the caspase-3 pathway. Our results show that caspase-3 may play an important role in radiation-induced apoptosis through a p53-independent pathway.
英文关键词	C-12(6+) irradiation; cell cycle; apoptosis; caspase-3; H1299 (p53 null)
WOS研究方向	Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
WOS类目	Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
WOS关键词	PATHWAYS ; DEATH ; ION
WOS记录号	WOS:000301440900002
来源期刊	CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
来源机构	中国科学院西北生态环境资源研究院
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/240999
作者单位	[Xu, Hua; Gao, Lan; Che, Tuanjie; Gao, Qingxiang; Wang, Ziren] Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China; [Du, Wenjing] Gansu Acad Sci, Inst Biol, Lanzhou, Peoples R China; [Li, Qiang] Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Xu, Hua,Gao, Lan,Che, Tuanjie,et al. The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299[J]. 中国科学院西北生态环境资源研究院,2012,27(2).
APA	Xu, Hua.,Gao, Lan.,Che, Tuanjie.,Du, Wenjing.,Li, Qiang.,...&Wang, Ziren.(2012).The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,27(2).
MLA	Xu, Hua,et al."The Effects of C-12(6+) Irradiation on Cell Cycle, Apoptosis, and Expression of Caspase-3 in the Human Lung Cancer Cell Line H1299".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 27.2(2012).