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DOI	10.1073/pnas.2016391118
	Threshold accumulation of a constitutive protein explains E. coli cell-division behavior in nutrient upshifts
	Panlilio M.; Grilli J.; Tallarico G.; Iuliani I.; Sclavi B.; Cicuta P.; Lagomarsino M.C.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:18
英文摘要	Despite a boost of recent progress in dynamic single-cell measurements and analyses in Escherichia coli, we still lack a mechanistic understanding of the determinants of the decision to divide. Specifically, the debate is open regarding the processes linking growth and chromosome replication to division and on the molecular origin of the observed “adder correlations,” whereby cells divide, adding roughly a constant volume independent of their initial volume. In order to gain insight into these questions, we interrogate dynamic size-growth behavior of single cells across nutrient upshifts with a high-precision microfluidic device. We find that the division rate changes quickly after nutrients change, much before growth rate goes to a steady state, and in a way that adder correlations are robustly conserved. Comparison of these data to simple mathematical models falsifies proposed mechanisms, where replication-segregation or septum completions are the limiting step for cell division. Instead, we show that the accumulation of a putative constitutively expressed “P-sector divisor” protein explains the behavior during the shift. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Cell division; Cell growth; E. coli; Mathematical modeling; Single-cell biology
语种	英语
scopus关键词	article; cell division; cell growth; cytology; Escherichia coli; growth rate; nonhuman; nutrient; steady state
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238926
作者单位	Cavendish Laboratory, Cambridge University, Cambridge, CB2 1TN, United Kingdom; Quantitative Life Sciences, The Abdus Salam International Centre for Theoretical Physics, Trieste, 34151, Italy; Dipartimento di Fisica, Università degli Studi di Milano, Milan, 20133, Italy; Istituto FIRC di Oncologia Molecolare (IFOM) Foundation, Fondazione Italiana per la Ricerca sul Cancro (FIRC) Institute of Molecular Oncology, Milan, 20139, Italy; Campus Pierre and Marie Curie, Sorbonne Université, Paris, 75005, France; Laboratory of Computational and Quantitative Biology, UMR 7238, CNRS, Paris, 75005, France; Laboratoire de Biologie et Pharmacologie Appliquée, UMR 8113 CNRS, Gif-sur-Yvette, 91190, France; École Normale Supérieure (ENS) Paris-Saclay, Gif-sur-Yvette, 91190, France; Istituto Nazionale di Fisica Nucleare, Milan, 20133, Italy
推荐引用方式 GB/T 7714	Panlilio M.,Grilli J.,Tallarico G.,et al. Threshold accumulation of a constitutive protein explains E. coli cell-division behavior in nutrient upshifts[J],2021,118(18).
APA	Panlilio M..,Grilli J..,Tallarico G..,Iuliani I..,Sclavi B..,...&Lagomarsino M.C..(2021).Threshold accumulation of a constitutive protein explains E. coli cell-division behavior in nutrient upshifts.Proceedings of the National Academy of Sciences of the United States of America,118(18).
MLA	Panlilio M.,et al."Threshold accumulation of a constitutive protein explains E. coli cell-division behavior in nutrient upshifts".Proceedings of the National Academy of Sciences of the United States of America 118.18(2021).