气候变化领域集成服务门户(CCPortal): Attaining the promise of plant gene editing at scale

CCPortal

DOI	10.1073/pnas.2004846117
	Attaining the promise of plant gene editing at scale
	Nasti R.A.; Voytas D.F.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:22
英文摘要	Crop improvement relies heavily on genetic variation that arises spontaneously through mutation. Modern breeding methods are very adept at combining this genetic variation in ways that achieve remarkable improvements in plant performance. Novel traits have also been created through mutation breeding and transgenesis. The advent of gene editing, however, marks a turning point: With gene editing, synthetic variation will increasingly supplement and, in some cases, supplant the genetic variation that occurs naturally. We are still in the very early stages of realizing the opportunity provided by plant gene editing. At present, typically only one or a few genes are targeted for mutation at a time, and most mutations result in loss of gene function. New technological developments, however, promise to make it possible to perform gene editing at scale. RNA virus vectors, for example, can deliver gene-editing reagents to the germ line through infection and create hundreds to thousands of diverse mutations in the progeny of infected plants. With developmental regulators, edited somatic cells can be induced to form meristems that yield seed-producing shoots, thereby increasing throughput and shrinking timescales for creating edited plants. As these approaches are refined and others developed, they will allow for accelerated breeding, the domestication of orphan crops and the reengineering of metabolism in a more directed manner than has ever previously been possible. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Crop improvement; Gene editing; Plant breeding
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238902
作者单位	Department of Genetics, Cell Biology and Development, Center for Genome Engineering, Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN 55108, United States
推荐引用方式 GB/T 7714	Nasti R.A.,Voytas D.F.. Attaining the promise of plant gene editing at scale[J],2021,118(22).
APA	Nasti R.A.,&Voytas D.F..(2021).Attaining the promise of plant gene editing at scale.Proceedings of the National Academy of Sciences of the United States of America,118(22).
MLA	Nasti R.A.,et al."Attaining the promise of plant gene editing at scale".Proceedings of the National Academy of Sciences of the United States of America 118.22(2021).