气候变化领域集成服务门户(CCPortal): Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae

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DOI	10.1073/pnas.2104813118
	Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae
	Manera K.; Caro F.; Li H.; Pei T.-T.; Hersch S.J.; Mekalanos J.J.; Dong T.G.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:25
英文摘要	The type 6 secretion system (T6SS) is a bacterial weapon broadly distributed in gram-negative bacteria and used to kill competitors and predators. Featuring a long and double-tubular structure, this molecular machine is energetically costly to produce and thus is likely subject to diverse regulation strategies that are largely ill defined. In this study, we report a quantity-sensing control of the T6SS that down-regulates the expression of secreted components when they accumulate in the cytosol due to T6SS inactivation. Using Vibrio cholerae strains that constitutively express an active T6SS, we demonstrate that mRNA levels of secreted components, including the inner-tube protein component Hcp, were down-regulated in T6SS structural gene mutants while expression of the main structural genes remained unchanged. Deletion of both hcp gene copies restored expression from their promoters, while Hcp overexpression negatively impacted expression. We show that Hcp directly interacts with the RpoN-dependent T6SS regulator VasH, and deleting the N-terminal regulator domain of VasH abolishes this interaction as well as the expression difference of hcp operons between T6SS-active and inactive strains. We find that negative regulation of hcp also occurs in other V. cholerae strains and the pathogens Aeromonas dhakensis and Pseudomonas aeruginosa. This Hcp-dependent sensing control is likely an important energy-conserving mechanism that enables T6SS-encoding organisms to quickly adjust T6SS expression and prevent wasteful build-up of its major secreted components in the absence of their efficient export out of the bacterial cell. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Vibriocholerae \| type 6 secretion system \| gene regulation
语种	英语
scopus关键词	bacterial protein; Hcp protein; messenger RNA; sigma factor RpoN; unclassified drug; Aeromonas; Aeromonas dhakensis; Article; bacterial cell; controlled study; cytosol; down regulation; energy conservation; gene deletion; gene mutation; nonhuman; operon; promoter region; protein domain; protein expression; protein interaction; protein secretion; protein transport; Pseudomonas aeruginosa; quorum sensing; strain difference; structural gene; type VI secretion system; Vibrio cholerae
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238889
作者单位	Department of Ecosystem and Public Health, University of Calgary, Calgary, AB T2N 4Z6, Canada; Department of Microbiology, Harvard Medical School, Boston, MA 02115, United States; State Key Laboratory of Microbial Metabolism, Joint International Laboratory on Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
推荐引用方式 GB/T 7714	Manera K.,Caro F.,Li H.,et al. Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae[J],2021,118(25).
APA	Manera K..,Caro F..,Li H..,Pei T.-T..,Hersch S.J..,...&Dong T.G..(2021).Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae.Proceedings of the National Academy of Sciences of the United States of America,118(25).
MLA	Manera K.,et al."Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae".Proceedings of the National Academy of Sciences of the United States of America 118.25(2021).