气候变化领域集成服务门户(CCPortal): The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection

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DOI	10.1073/pnas.2026806118
	The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection
	Kim S.; Park J.-S.; Lee J.; Lee K.K.; Park O.-S.; Choi H.-S.; Seo P.J.; Cho H.-T.; Frost J.M.; Fischer R.L.; Choi Y.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:29
英文摘要	The flowering plant life cycle consists of alternating haploid (gametophyte) and diploid (sporophyte) generations, where the sporophytic generation begins with fertilization of haploid gametes. In Arabidopsis, genome-wide DNA demethylation is required for normal development, catalyzed by the DEMETER (DME) DNA demethylase in the gamete companion cells of male and female gametophytes. In the sporophyte, postembryonic growth and development are largely dependent on the activity of numerous stem cell niches, or meristems. Analyzing Arabidopsis plants homozygous for a loss-of-function dme-2 allele, we show that DME influences many aspects of sporophytic growth and development. dme-2 mutants exhibited delayed seed germination, variable root hair growth, aberrant cellular proliferation and differentiation followed by enhanced de novo shoot formation, dysregulation of root quiescence and stomatal precursor cells, and inflorescence meristem (IM) resurrection. We also show that sporophytic DME activity exerts a profound effect on the transcriptome of developing Arabidopsis plants, including discrete groups of regulatory genes that are misregulated in dme-2 mutant tissues, allowing us to potentially link phenotypes to changes in specific gene expression pathways. These results show that DME plays a key role in sporophytic development and suggest that DME-mediated active DNA demethylation may be involved in the maintenance of stem cell activities during the sporophytic life cycle in Arabidopsis. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	DNA demethylation \| sprorophytic development \| cell proliferation \| pluripotency
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238858
作者单位	Department of Biological Sciences, Seoul National University, Seoul, 08826, South Korea; Department of Chemistry, Seoul National University, Seoul, 08826, South Korea; Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, United States
推荐引用方式 GB/T 7714	Kim S.,Park J.-S.,Lee J.,et al. The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection[J],2021,118(29).
APA	Kim S..,Park J.-S..,Lee J..,Lee K.K..,Park O.-S..,...&Choi Y..(2021).The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection.Proceedings of the National Academy of Sciences of the United States of America,118(29).
MLA	Kim S.,et al."The DME demethylase regulates sporophyte gene expression, cell proliferation, differentiation, and meristem resurrection".Proceedings of the National Academy of Sciences of the United States of America 118.29(2021).