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DOI	10.1073/pnas.2107065118
	Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk
	Kono N.; Nakamura H.; Mori M.; Yoshida Y.; Ohtoshi R.; Malay A.D.; Pedrazzoli Moran D.A.; Tomita M.; Numata K.; Arakawa K.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:31
英文摘要	Dragline silk of golden orb-weaver spiders (Nephilinae) is noted for its unsurpassed toughness, combining extraordinary extensibility and tensile strength, suggesting industrial application as a sustainable biopolymer material. To pinpoint the molecular composition of dragline silk and the roles of its constituents in achieving its mechanical properties, we report a multiomics approach, combining highquality genome sequencing and assembly, silk gland transcriptomics, and dragline silk proteomics of four Nephilinae spiders.We observed the consistent presence of the MaSp3B spidroin unique to this subfamily as well as several nonspidroin SpiCE proteins. Artificial synthesis and the combination of these components in vitro showed that the multicomponent nature of dragline silk, including MaSp3B and SpiCE, along with MaSp1 and MaSp2, is essential to realize the mechanical properties of spider dragline silk. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Genome; Mechanical property; Orb-weaving spider; Spider silk
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238843
作者单位	Institute for Advanced Biosciences, Keio University, 403-1 Nihonkoku, Tsuruoka, Yamagata, Daihouji, 997-0017, Japan; Systems Biology Program, Graduate School of Media and Governance, Keio University, 5322 Endo, Kanagawa, Fujisawa, 252-0882, Japan; Spiber Inc., 234-1 Mizukami, Tsuruoka, Yamagata, Kakuganji, 997-0052, Japan; Center for Sustainable Resource Science, RIKEN, 2-1 Hirosawa, Saitama, Wako, 351-0198, Japan; Depertment of Material Chemistry, Kyoto University, Nishikyo-ku, Kyoto, Kyotodaigaku-Katsura, 615-8510, Japan
推荐引用方式 GB/T 7714	Kono N.,Nakamura H.,Mori M.,et al. Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk[J],2021,118(31).
APA	Kono N..,Nakamura H..,Mori M..,Yoshida Y..,Ohtoshi R..,...&Arakawa K..(2021).Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk.Proceedings of the National Academy of Sciences of the United States of America,118(31).
MLA	Kono N.,et al."Multicomponent nature underlies the extraordinary mechanical properties of spider dragline silk".Proceedings of the National Academy of Sciences of the United States of America 118.31(2021).