气候变化领域集成服务门户(CCPortal): Structural analysis of receptors and actin polarity in platelet protrusions

CCPortal

DOI	10.1073/pnas.2105004118
	Structural analysis of receptors and actin polarity in platelet protrusions
	Sorrentino S.; Conesa J.J.; Cuervo A.; Melero R.; Martins B.; Fernandez-Gimenez E.; De Isidro-Gomez F.P.; De la Morena J.; Studt J.-D.; Sorzano C.O.S.; Eibauer M.; Carazo J.M.; Medalia O.
发表日期	2021
ISSN	0027-8424
卷号	118 期号:37
英文摘要	During activation the platelet cytoskeleton is reorganized, inducing adhesion to the extracellular matrix and cell spreading. These processes are critical for wound healing and clot formation. Initially, this task relies on the formation of strong cellular-extracellular matrix interactions, exposed in subendothelial lesions. Despite the medical relevance of these processes, there is a lack of high-resolution structural information on the platelet cytoskeleton controlling cell spreading and adhesion. Here, we present in situ structural analysis of membrane receptors and the underlying cytoskeleton in platelet protrusions by applying cryoelectron tomography to intact platelets. We utilized three-dimensional averaging procedures to study receptors at the plasma membrane. Analysis of substrate interaction-free receptors yielded one main structural class resolved to 26 Å, resembling the αIIbβ3 integrin folded conformation. Furthermore, structural analysis of the actin network in pseudopodia indicates a nonuniform polarity of filaments. This organization would allow generation of the contractile forces required for integrin-mediated cell adhesion. © 2021 National Academy of Sciences. All rights reserved.
英文关键词	Actin; Cryoelectron tomography; Platelets; Receptors
语种	英语
来源期刊	Proceedings of the National Academy of Sciences of the United States of America
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/238800
作者单位	Biochemistry Department, University of Zurich, Zurich, 8006, Switzerland; Instruct Image Processing Center, National Center of Biotechnology, Madrid, 28049, Spain; Division of Hematology, University Hospital Zurich, Zurich, 8091, Switzerland
推荐引用方式 GB/T 7714	Sorrentino S.,Conesa J.J.,Cuervo A.,et al. Structural analysis of receptors and actin polarity in platelet protrusions[J],2021,118(37).
APA	Sorrentino S..,Conesa J.J..,Cuervo A..,Melero R..,Martins B..,...&Medalia O..(2021).Structural analysis of receptors and actin polarity in platelet protrusions.Proceedings of the National Academy of Sciences of the United States of America,118(37).
MLA	Sorrentino S.,et al."Structural analysis of receptors and actin polarity in platelet protrusions".Proceedings of the National Academy of Sciences of the United States of America 118.37(2021).